CXCR4 Recombinant Rabbit Monoclonal Antibody [PSH22-76]
cat.: HA724321
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | PSH22-76 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Positive control: | Jurkat (Human T-lymphoblastic cells) cell lysate, HepG2 (Human liver cancer cells) cell lysate, Ramos (Human Burkitt's lymphoma cells) cell lysate, Daudi (Human Burkitt's lymphoma cells) cell lysate. |
| Subcellular location: | Cell membrane, Cell junction, Early endosome, Late endosome, Lysosome. |
| Recommended Dilutions:
WB IHC-Fr |
1:5,000 1:500 |
| Uniprot #: | SwissProt: P61073 Human | P70658 Mouse |
| Alternative names: | C-X-C chemokine receptor type 4 CD184 CD184 antigen Chemokine (C X C motif) receptor 4 Chemokine CXC Motif Receptor 4 CXC-R4 CXCR-4 CXCR4 CXCR4_HUMAN D2S201E FB22 Fusin HM89 HSY3RR LAP 3 LAP3 LCR1 LESTR Leukocyte derived seven transmembrane domain receptor Leukocyte-derived seven transmembrane domain receptor Lipopolysaccharide associated protein 3 Neuropeptide Y receptor Y3 NPY3R NPYR NPYRL NPYY3 NPYY3R Probable G protein coupled receptor lcr1 homolog SDF 1 receptor SDF-1 receptor SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR Stromal cell derived factor 1 receptor Stromal cell-derived factor 1 receptor WHIM WHIMS |
Images
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Fig1:
Western blot analysis of CXCR4 on different lysates with Rabbit anti-CXCR4 antibody (HA724321) at 1/5,000 dilution. Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate (Unboiled) Lane 2: HepG2 (Human liver cancer cells) cell lysate (negative) Lane 3: Ramos (Human Burkitt's lymphoma cells) cell lysate (Unboiled) Lane 4: Daudi (Human Burkitt's lymphoma cells) cell lysate (Unboiled) Lysates/proteins at 15 µg/Lane. Exposure time: 6 seconds; ECL: K1801 HepG2 is a negative control (PMID: 25363530). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724321, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 40 kDa Observed band size: 30-50 kDa |
|
Fig2:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: E14.0 embryonic brain Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× PBS Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature. Primary antibody: HA724321, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.