IL-6R Recombinant Rabbit Monoclonal Antibody [PSH22-78]
cat.: HA724323
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH22-78 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IL-6R aa 1-386. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB FC |
1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P08887 Human |
| Alternative names: | CD 126 CD126 CD126 antigen gp80 IL 6R 1 IL 6R alpha IL 6R IL-6 receptor alpha chain IL-6 receptor subunit alpha IL-6R 1 IL-6R subunit alpha IL-6R-alpha IL-6RA IL6Q Il6r IL6RA IL6RA_HUMAN IL6RQ Interleukin 6 receptor interleukin 6 receptor, alpha Interleukin-6 receptor subunit alpha Membrane glycoprotein 80 MGC30256 |
Images
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Fig1:
Western blot analysis of IL-6R on different lysates with Rabbit anti-IL-6R antibody (HA724323) at 1/2,000 dilution.
Lane 1: THP-1 (Human acute monoblastic leukemia cells) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 3: U-937 (Human acute monocytic leukemia cells) cell lysate Lane 4: Daudi (Human Burkitt's lymphoma cells) cell lysate Lane 5: U266 (Human multiple myeloma cell) cell lysate Lane 6: K-562 (Human chronic myelogenous leukemia cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Negative control: K-562 and Daudi (PMID : 8300137). Negative expression of IL-6R protein in HeLa cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724323, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52 kDa Observed band size: 70-100 kDa |
|
Fig2:
Application: Flow Cytometry
Species: Human Sample: THP-1 (Human acute monoblastic leukemia cells) Blocking: 1% BSA + 10% normal goat serum + human FcR (Miltenyi Biotec), 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724323 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. Negative expression of IL-6R protein in HeLa cell is consistent with the predicted expression pattern. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.