Human CD40 Recombinant Rabbit Monoclonal Antibody [PSH22-79]
cat.: HA724324
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH22-79 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD40 aa 21-193 (HA211232). |
| Positive control: | Recombinant Human CD40 protein (HA211232). |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-80] to Human CD40 (Detecor) (HA724325) and Recombinant Human CD40 protein (HA211232) as the standard. The reference range value is 3.9-1,000 pg/mL. |
| Uniprot #: | SwissProt: P25942 Human |
| Alternative names: | AI326936 B cell associated molecule CD40 B cell surface antigen CD40 B cell-associated molecule B-cell surface antigen CD40 Bp50 CD 40 CD40 CD40 antigen (TNF receptor superfamily member 5) CD40 antigen CD40 molecule CD40 molecule, TNF receptor superfamily member 5 CD40 protein CD40 type II isoform CD40L receptor CDw40 GP39 HIGM1 IGM IMD3 MGC9013 Nerve growth factor receptor related B lymphocyte activation molecule OTTHUMP00000031699 OTTHUMP00000031700 p50 T-BAM TBAM TNF receptor superfamily member 5 TNFRSF5 TNR5_HUMAN TRAP Tumor necrosis factor receptor superfamily , member 5 Tumor necrosis factor receptor superfamily member 5 Tumor necrosis factor receptor superfamily member 5 precursor Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a |
Images
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Fig1:
Sandwich ELISA analysis of Human CD40 matched pair antibodies Capture: HA724324, Human CD40 Rabbit mAb [PSH22-79] Detector: HA724325, Human CD40 Rabbit mAb [PSH22-80] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724324) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD40 protein (HA211232) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA724325, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CD14 in Human serum. Capture: HA724324, Human CD40 Rabbit mAb [PSH22-79] Detector: HA724325, Human CD40 Rabbit mAb [PSH22-80] Interpolated concentration of native CD14 was measured in duplicate at different sample concentrations and interpolated from the CD14 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD14 concentration was determined to be 808 pg/ml in Human serum. |
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Fig3:
Interpolated concentrations of native CD40 in human samples. Capture: HA724324, Human CD40 Rabbit mAb [PSH22-79] Detector: HA724325, Human CD40 Rabbit mAb [PSH22-80] Interpolated concentration of native CD40 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD40 concentration was determined to be 1,566 pg/mL in human urine and 41 pg/ml in Ramos cell culture supernatant.There was no detectable signal in Jurkat cell culture supernatant. |
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Fig4:
Interpolated concentrations of spiked CD40 in cell culture media samples. Capture: HA724324, Human CD40 Rabbit mAb [PSH22-79] Detector: HA724325, Human CD40 Rabbit mAb [PSH22-80] The concentrations of CD40 were measured in duplicates, interpolated from the CD40 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.