Biotin Conjugated Human IL-18R1 Recombinant Rabbit Monoclonal Antibody [PSH22-86]
cat.: HA724331B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH22-86 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL-18R1 aa 22-329 (HA211256). |
| Positive control: | Recombinant Human IL-18R1 protein (HA211256). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-85] to Human IL-18R1 antibody (Capture) (HA724330) and Recombinant Human IL-18R1 protein (HA211256) as the standard. The reference range value is 11.7-3,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q13478 Human |
| Alternative names: | CD218 antigen-like family member A CD218a CDw218a CDw218a antigen IL 1Rrp IL-18R-1 IL-18R1 IL-1Rrp IL1 receptor related protein IL1 receptor-related protein IL18R_HUMAN IL18R1 IL18RA Il18ralpha IL1R-rp IL1RRP Interleukin 18 receptor 1 Interleukin 18 receptor alpha chain Interleukin-18 receptor 1 |
Images
|
Fig1:
Sandwich ELISA analysis of Human IL-18R1 matched pair antibodies Capture: HA724330, Human IL-18R1 Rabbit mAb [PSH22-85] Detector: HA724331, Human IL-18R1 Rabbit mAb [PSH22-86] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724330) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-18R1 protein (HA211256) starting from 3000 pg/ml to 0 pg/ml and detect antibody (HA724331, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native IL-18R1 in NK-92, U-937 and THP-1 cell extract samples based on a 1000 µg/ml extract load. Capture: HA724330, Human IL-18R1 Rabbit mAb [PSH22-85] Detector: HA724331, Human IL-18R1 Rabbit mAb [PSH22-86] Interpolated concentration of native IL-18R1 was measured in duplicate at different sample concentrations and interpolated from the IL-18R1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-18R1 concentration was determined to be 3,518 pg/mL in NK-92 and 891 pg/mL in U-937 cell extract. There was no detectable signal in THP-1 cell extract. |
|
Fig3:
Interpolated concentrations of spiked IL-18R1 in cell culture media samples. Capture: HA724330, Human IL-18R1 Rabbit mAb [PSH22-85] Detector: HA724331, Human IL-18R1 Rabbit mAb [PSH22-86] The concentrations of IL-18R1 were measured in duplicates, interpolated from the IL-18R1 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.