Mouse/Rat IGFBP3 Recombinant Rabbit Monoclonal Antibody [PSH22-89]
cat.: HA724334
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH22-89 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse IGFBP3 aa 28-292 (HA211243). |
| Positive control: | Recombinant Mouse IGFBP3 protein (HA211243). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-90] to Mouse/Rat IGFBP3 antibody (Detector) (HA724335) and Recombinant Mouse IGFBP3 protein (HA211243) as the standard. The reference range value is 39.1-10,000 pg/mL. |
| Uniprot #: | SwissProt: P47878 Mouse | P15473 Rat |
| Alternative names: | Acid stable subunit of the 140 K IGF complex Binding protein 29 Binding protein 53 BP 53 BP53 Growth hormone dependent binding protein IBP 3 IBP-3 IBP3 IGF binding protein 3 IGF-binding protein 3 IGFBP 3 IGFBP-3 IGFBP3 Insulin Like Growth Factor Binding Protein 3 Insulin-like growth factor binding protein 3 precursor Insulin-like growth factor-binding protein 3 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse IGFBP3 matched pair antibodies Capture: HA724334, Mouse/Rat IGFBP3 Rabbit mAb [PSH22-89] Detector: HA724335, Mouse/Rat IGFBP3 Rabbit mAb [PSH22-90] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724334) diluted in carbonate/bicarbonate buffer, at a concentMouseion of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.2% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IGFBP3 protein (HA211243) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724335, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB SubstMousee for 10 minutes at room tempeMouseure in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native IGFBP3 in mouse serum samples. Capture: HA724334, Mouse/Rat IGFBP3 Rabbit mAb [PSH22-89] Detector: HA724335, Mouse/Rat IGFBP3 Rabbit mAb [PSH22-90] The concentrations of IGFBP3 were measured in duplicates, interpolated from the IGFBP3 standard curve and corrected for sample dilution. Undiluted samples are mouse serum 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 861.2 ng/ml in mouse serum. |
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Fig3:
Interpolated concentrations of native IGFBP3 in rat serum samples. Capture: HA724334, Mouse/Rat IGFBP3 Rabbit mAb [PSH22-89] Detector: HA724335, Mouse/Rat IGFBP3 Rabbit mAb [PSH22-90] The concentrations of IGFBP3 were measured in duplicates, interpolated from the IGFBP3 standard curve and corrected for sample dilution. Undiluted samples are rat serum 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 256.7 ng/ml in rat serum. |
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Fig4:
Interpolated concentrations of spiked IGFBP3 in mouse cell culture media samples. Capture: HA724334, Mouse/Rat IGFBP3 Rabbit mAb [PSH22-89] Detector: HA724335, Mouse/Rat IGFBP3 Rabbit mAb [PSH22-90] The concentrations of IGFBP3 were measured in duplicates, interpolated from the IGFBP3 standard curves and corrected for sample dilution. Diluted samples are as follows: 25% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.