LDHB Recombinant Rabbit Monoclonal Antibody [PSH22-91]
cat.: HA724336
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey, Pig |
| Applications: | WB, IF-Cell, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH22-91 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human LDHB aa 1-334. |
| Positive control: | HEK-293 (Human embryonic kidney cells) cell lysate, Huh7 (Human liver cancer cells) cell lysate, LNCaP (Human prostate cancer cells) cell lysate, HeLa (Human cervical adenocarcinoma cells) cell lysate, COS-1 (African green monkey kidney fibroblasts) cell lysate, PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate, Mouse heart tissue lysate, Mouse kidney tissue lysate, Mouse brain tissue lysate, Rat heart tissue lysate, Rat kidney tissue lysate, Rat brain tissue lysate, Pig brain tissue lysate. |
| Subcellular location: | Cytoplasm, Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IF-Cell FC IP |
1:20,000-1:50,000 1:500 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P07195 Human | P16125 Mouse | P42123 Rat |
| Alternative names: | Epididymis secretory protein Li 281 HEL S 281 L lactate dehydrogenase B chain L-lactate dehydrogenase B chain Lactate dehydrogenase H chain LDH B LDH H LDH heart subunit LDH-B LDH-H LDHB LDHB_HUMAN LDHBD LDHH Renal carcinoma antigen NY REN 46 Renal carcinoma antigen NY-REN-46 TRG-5 TRG5 |
Images
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Fig1:
Western blot analysis of LDHB on different lysates with Rabbit anti-LDHB antibody (HA724336) at 1/20,000 dilution.
Lane 1: HEK-293 (Human embryonic kidney cells) cell lysate Lane 2: Huh7 (Human liver cancer cells) cell lysate Lane 3: LNCaP (Human prostate cancer cells) cell lysate Lane 4: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 5: COS-1 (African green monkey kidney fibroblasts) cell lysate Lane 6: PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 21 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724336, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 37 kDa Observed band size: 37 kDa |
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Fig2:
Western blot analysis of LDHB on different lysates with Rabbit anti-LDHB antibody (HA724336) at 1/20,000 dilution.
Lane 1: Mouse heart tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Mouse brain tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724336, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 37 kDa Observed band size: 37 kDa |
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Fig3:
Western blot analysis of LDHB on different lysates with Rabbit anti-LDHB antibody (HA724336) at 1/50,000 dilution.
Lane 1: Rat heart tissue lysate Lane 2: Rat kidney tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Pig brain tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 4 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724336, 1/50,000 in 5% NFDM/TBST, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 37 kDa Observed band size: 37 kDa |
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Fig4:
Application: Immunocytochemistry (IF-cell)
Species: Rat Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated)) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724336, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). |
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Fig5:
Application: Flow Cytometry (Intra)
Species: Rat Sample: PC-12 (Rat pheochromocytoma cells (undifferentiated)) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724336 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
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Fig6:
Immunoprecipitation (IP) LDHB was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with HA724336 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724336 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA724336 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA724336 in HeLa cell lysate Exposure time: 2 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724336, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 37 kDa Observed band size: 37 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.