Wilms Tumor Protein Recombinant Rabbit Monoclonal Antibody [PSH23-02]
cat.: HA724341
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH23-02 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG |
| Positive control: | Human Testis, Human Ovarian borderline serous carcinoma, Human Kidney, Human Mesothelioma. |
| Subcellular location: | Nucleus, Cytoplasm, Nucleus speckle. |
| Recommended Dilutions:
IHC-P |
1:200-1:1,000 |
| Uniprot #: | SwissProt: P19544 Human |
| Alternative names: | WIT 2 WT 1 AWT1 FWT1 GUD NPHS4 WAGR Wilms tumor 1 Wilms Tumor Wilms tumor protein Wilms' tumor gene Wilms' tumor protein WIT2 WT WT1 WT1_HUMAN WT33 |
Images
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Fig1:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Testis Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724341, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Ovarian borderline serous carcinoma Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724341, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724341, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Mesothelioma Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724341, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Lung adenocarcinoma (Negative control) Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724341, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Clear cell renal cell carcinoma (Negative control) Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724341, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig7:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Breast carcinoma (Negative control) Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724341, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig8:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Colorectal carcinoma (Negative control) Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H2O2, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724341, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.