MLH1 Recombinant Antibody [PSH20-67] - Rabbit IgG (Chimeric)
cat.: HA724342
| Product Type: | Recombinant Chimeric Antibody, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Cell, WB |
| Clone number: | PSH20-67 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 85 kDa |
| Positive control: | HeLa (Human cervical adenocarcinoma cells) cell lysate, SW480 (Human colorectal zdenocarcinoma cells) cell lysate. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
IHC-P IF-Cell WB |
1:2,000 1:3,000 1:2,000 |
| Uniprot #: | SwissProt: P40692 Human |
| Alternative names: | COCA 2 COCA2 DNA mismatch repair protein Mlh1 FCC 2 FCC2 hMLH 1 hMLH1 HNPCC 2 HNPCC HNPCC2 MGC5172 MLH 1 MLH1 MLH1_HUMAN MutL homolog 1 (E. coli) MutL homolog 1 MutL homolog 1 colon cancer nonpolyposis type 2 MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) MutL protein homolog 1 MutL, E. coli, homolog of, 1 |
Images
|
Fig1:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0) (high pressure), 2 minutes. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724342, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Appendix Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0) (high pressure), 2 minutes. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724342, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunocytochemistry (IF-cell) Species: Human Sample: SW480 (Human colorectal zdenocarcinoma cells)/HCT 116 (Human colon cancer cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724342, 1/3,000, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). Negative control: HCT 116 (PMID: 27618981). |
|
Fig4:
Western blot analysis of MLH1 on different lysates with Rabbit anti-MLH1 antibody (HA724342) at 1/2,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 2: HCT 116 (Human colon cancer cells) cell lysate (negative) Lane 3: SW480 (Human colorectal zdenocarcinoma cells) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 12 seconds; ECL: K1801 HCT 116 is a negative control (PMID: 34787019). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724342, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 85 kDa Observed band size: 85 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.