EPS15 Recombinant Rabbit Monoclonal Antibody [PSH23-03]
cat.: HA724343
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH23-03 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 99 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human EPS15 aa 490-896. |
| Positive control: | A431 (Human epidermoid carcinoma skin squamous cells) cell lysate, HeLa (Human cervical adenocarcinoma cells) cell lysate, Huh7 (Human liver cancer cells) cell lysate, A20 (Mouse B-cell lymphoma cells) cell lysate, PC-12 (Rat pheochromocytoma cells ) cell lysate, COS-1 (African green monkey kidney fibroblasts) cell lysate. |
| Subcellular location: | Cytoplasm, Cell membrane, Membrane, clathrin-coated pit; Early endosome membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:20,000 1:200 1:2,000 |
| Uniprot #: | SwissProt: P42566 Human | P42567 Mouse Entrez Gene: 313474 Rat |
| Alternative names: | Epidermal growth factor receptor substrate 15 Protein Eps15 Protein AF-1p EPS15 AF1P |
Images
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Fig1:
Western blot analysis of EPS15 on different lysates with Rabbit anti-EPS15 antibody (HA724343) at 1/20,000 dilution.
Lane 1: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 3: Huh7 (Human liver cancer cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 12 seconds; ECL: K1801 Molecular weight（PMID: 18362181, PMID: 7689153) Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724343, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 99 kDa Observed band size: 138 kDa |
|
Fig2:
Western blot analysis of EPS15 on different lysates with Rabbit anti-EPS15 antibody (HA724343) at 1/20,000 dilution.
Lane 1: A20 (Mouse B-cell lymphoma cells) cell lysate Lane 2: PC-12 (Rat pheochromocytoma cells ) cell lysate Lane 3: COS-1 (African green monkey kidney fibroblasts) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Molecular weight（PMID: 18362181, PMID: 7689153) Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724343, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 99 kDa Observed band size: 138 kDa |
|
Fig3:
Application: Immunocytochemistry (IF-cell)
Species: Mouse Sample: A20 (Mouse B-cell lymphoma cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724343, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). |
|
Fig4:
Application: Flow Cytometry (Intra)
Species: Mouse Sample: A20 (Mouse B-cell lymphoma cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724343 (1/2,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.