Mouse/Rat Alpha-synuclein Recombinant Rabbit Monoclonal Antibody [PSH23-05]
cat.: HA724345
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH23-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse Alpha-synuclein aa 1-140 (HA211105). |
| Positive control: | Recombinant Mouse Alpha-synuclein protein (HA211105). |
| Subcellular location: | Cytoplasm, Membrane, Nucleus, Synapse, Secreted, Cell projection, axon. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-06] to Mouse/Rat Alpha-synuclein (Detecor) (HA724346) and Recombinant Mouse Alpha-synuclein protein (HA211105) as the standard. The reference range value is 19.5-5,000 pg/mL. |
| Uniprot #: | SwissProt: O55042 Mouse | P37377 Rat |
| Alternative names: | Alpha synuclein Alpha-synuclein Alpha-synuclein, isoform NACP140 alphaSYN MGC105443 MGC110988 MGC127560 MGC64356 NACP Non A beta component of AD amyloid Non A4 component of amyloid Non A4 component of amyloid precursor Non-A beta component of AD amyloid Non-A-beta component of alzheimers disease amyloid , precursor of Non-A4 component of amyloid precursor Non-A4 component of amyloid, precursor of OTTHUMP00000218549 OTTHUMP00000218551 OTTHUMP00000218552 OTTHUMP00000218553 OTTHUMP00000218554 PARK 1 PARK 4 PARK1 PARK4 Parkinson disease (autosomal dominant, Lewy body) 4 Parkinson disease familial 1 SNCA Snca synuclein Snca synuclein, alpha (non A4 component of amyloid precursor) SYN Synuclein alpha Synuclein alpha 140 Synuclein, alpha (non A4 component of amyloid precursor) SYUA_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of Mouse/Rat Alpha-synuclein matched pair antibodies Capture: HA724345, Mouse/Rat Alpha-synuclein Rabbit mAb [PSH23-05] Detector: HA724346, Mouse/Rat Alpha-synuclein Rabbit mAb [PSH23-06] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724345) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Alpha-synuclein protein (HA211105) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA724346, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Alpha-synuclein in mouse samples. Capture: HA724345, Mouse/Rat Alpha-synuclein Rabbit mAb [PSH23-05] Detector: HA724346, Mouse/Rat Alpha-synuclein Rabbit mAb [PSH23-06] Interpolated concentration of native Alpha-synuclein was measured in duplicate at different sample concentrations and interpolated from the Alpha-synuclein standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Alpha-synuclein concentration was determined to be 167,105 pg/mL in mouse serum. |
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Fig3:
Interpolated concentrations of native Alpha-synuclein in rat samples. Capture: HA724345, Mouse/Rat Alpha-synuclein Rabbit mAb [PSH23-05] Detector: HA724346, Mouse/Rat Alpha-synuclein Rabbit mAb [PSH23-06] Interpolated concentration of native Alpha-synuclein was measured in duplicate at different sample concentrations and interpolated from the Alpha-synuclein standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Alpha-synuclein concentration was determined to be 1,015 pg/mL in rat serum. |
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Fig4:
Interpolated concentrations of native Alpha-synuclein in mouse brain, rat brain and A20 extract samples based on a 1000 µg/ml extract load. Capture: HA724345, Mouse/Rat Alpha-synuclein Rabbit mAb [PSH23-05] Detector: HA724346, Mouse/Rat Alpha-synuclein Rabbit mAb [PSH23-06] Interpolated concentration of native Alpha-synuclein was measured in duplicate at different sample concentrations and interpolated from the Alpha-synuclein standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Alpha-synuclein concentration was determined to be 1,049 ng/mL in mouse brain and 1,205 ng/mL in rat brain tissue extract, There was no detectable signal in A20 cell extract. |
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Fig5:
Interpolated concentrations of spiked Alpha-synuclein in cell culture media samples. Capture: HA724345, Mouse/Rat Alpha-synuclein Rabbit mAb [PSH23-05] Detector: HA724346, Mouse/Rat Alpha-synuclein Rabbit mAb [PSH23-06] The concentrations of Alpha-synuclein were measured in duplicates, interpolated from the Alpha-synuclein standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.