Hemopexin Recombinant Rabbit Monoclonal Antibody [PSH23-09]
cat.: HA724349
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: PSH23-09
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 52 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Hemopexin aa 1-462.
Subcellular location: Secreted.
Recommended Dilutions:
  WB

1:20,000
Uniprot #: SwissProt: P02790 Human | Q91X72 Mouse | P20059 Rat
Alternative names: Beta 1B glycoprotein Beta-1B-glycoprotein FLJ56652 HEMO HEMO_HUMAN Hemopexin Hpx HX
Images
HA724349_1.jpg Fig1: Western blot analysis of Hemopexin on different lysates with Rabbit anti-Hemopexin antibody (HA724349) at 1/20,000 dilution.

Lane 1: Mouse plasma
Lane 2: Mouse serum
Lane 3: Rat plasma

Lysates/proteins at 20 µg/Lane.
Exposure time: 12 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724349, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 52 kDa
Observed band size: 70 kDa
HA724349_2.jpg Fig2: Western blot analysis of Hemopexin on different lysates with Rabbit anti-Hemopexin antibody (HA724349) at 1/20,000 dilution.

Lane 1: Human plasma
Lane 2: Human serum

Lysates/proteins at 20 µg/Lane.
Exposure time: 3 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724349, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 52 kDa
Observed band size: 70 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.