Mouse CD27 Recombinant Rabbit Monoclonal Antibody [PSH23-17]
cat.: HA724352
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH23-17 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse CD27 aa 24-182 (HA211236). |
| Positive control: | Recombinant Mouse CD27 protein (HA211236). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-18] to Mouse CD27 (Detecor) (HA724353) and Recombinant Mouse CD27 protein (HA211236) as the standard. The reference range value is 39.1-5,000 pg/mL. |
| Uniprot #: | SwissProt: P41272 Mouse |
| Alternative names: | CD 27 CD27 CD27 antigen CD27 molecule CD27L receptor LPFS2 MGC20393 OTTHUMP00000238557 S152 T cell activation antigen CD27 T cell antivation antigen S152 T-cell activation antigen CD27 T14 TNFRSF 7 TNFRSF7 TNFSF7 Tp 55 Tp55 Tumor necrosis factor receptor superfamily member 7 |
Images
|
Fig1:
Sandwich ELISA analysis of Mouse CD27 matched pair antibodies Capture: HA724352, Mouse CD27 Rabbit mAb [PSH23-17] Detector: HA724353, Mouse CD27 Rabbit mAb [PSH23-18] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724352) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD27 protein (HA211236) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA724353, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native CD27 in Mouse serum. Capture: HA724352, Mouse CD27 Rabbit mAb [PSH23-17] Detector: HA724353, Mouse CD27 Rabbit mAb [PSH23-18] Interpolated concentration of native CD27 was measured in duplicate at different sample concentrations and interpolated from the CD27 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD27 concentration was determined to be 5,918 pg/ml in Mouse serum. |
|
Fig3:
Interpolated concentrations of native CD27 in mouse spleen and thymus tissue extract samples based on a 1000 µg/ml extract load. Capture: HA724352, Mouse CD27 Rabbit mAb [PSH23-17] Detector: HA724353, Mouse CD27 Rabbit mAb [PSH23-18] Interpolated concentration of native CD27 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD27 concentration was determined to be 2.903 pg/ml in mouse spleen tissue extract and 14,456 pg/ml in mouse thymus tissue extract. |
|
Fig4:
Interpolated concentrations of spiked CD27 in cell culture media samples. Capture: HA724352, Mouse CD27 Rabbit mAb [PSH23-17] Detector: HA724353, Mouse CD27 Rabbit mAb [PSH23-18] The concentrations of CD27 were measured in duplicates, interpolated from the CD27 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.