Granzyme K Recombinant Rabbit Monoclonal Antibody [PSH23-21]
cat.: HA724356
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH23-21 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Subcellular location: | Secreted, Cytoplasmic granule. |
| Recommended Dilutions:
WB IF-Cell IHC-P IF-Tissue |
1:5,000 1:100 1:100 1:100 |
| Uniprot #: | SwissProt: P49863 Human |
| Alternative names: | Fragmentin 3 Fragmentin-3 Fragmentin3 GRAK_HUMAN Granzyme 3 granzyme K (granzyme 3; tryptase II) granzyme K (serine protease, granzyme 3; tryptase II) Granzyme K Granzyme K precursor Granzyme-3 Granzyme3 GranzymeK GZMK NK TRYP 2 NK TRYP2 NK tryptase 2 NK-TRYP-2 NK-tryptase-2 NKTRYP2 Serine protease granzyme 3 TRYP 2 TRYP2 Tryptase II TryptaseII |
Images
|
Fig1:
Western blot analysis of Granzyme K on different lysates with Rabbit anti-Granzyme K antibody (HA724356) at 1/5,000 dilution.
Lane 1: NK-92 (Human natural killer cells) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Negative expression of Granzyme K protein in HeLa cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724356, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 29 kDa Observed band size: 17-27 kDa |
|
Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: NK-92 (Human natural killer cells)/HeLa (Human cervix adenocarcinoma epithelial cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724356, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). Negative expression of Granzyme K protein in HeLa cells is consistent with the predicted expression pattern. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: NK/T lymphoma Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× PBS Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724356, 1/100, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× PBS Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724356, 1/100, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Pancreas Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× PBS Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724356, 1/100, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of Granzyme K protein in pancreas is consistent with the predicted expression pattern. |
|
Fig6:
Application: Immunofluorescence (IF-tissue)
Species: Human Tissue: NK/T lymphoma Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× PBS Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724356, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.