FITC Conjugated S100 alpha 6 Recombinant Rabbit Monoclonal Antibody [JF0976]
cat.: HA724357F
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JF0976 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 10 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein of Human S100 alpha 6 aa 1-90 / 90. |
| Positive control: | C6, human kidney tissue. |
| Subcellular location: | Nucleus envelope, Cytoplasm, Cell membrane. |
| Recommended Dilutions:
IF-Cell IF-Tissue |
1:100 1:500 |
| Uniprot #: | SwissProt: P06703 Human | P14069 Mouse | P05964 Rat |
| Alternative names: | 2A9 5B10 CABP CACY Calcyclin Growth factor inducible protein 2A9 Growth factor-inducible protein 2A9 MLN 4 MLN4 OTTHUMP00000015472 OTTHUMP00000015473 PRA PRAGrowth factor inducible protein 2A9 Prolactin receptor associated protein Prolactin receptor-associated protein Protein S100 A6 Protein S100-A6 S100 A6 S100 calcium binding protein A6 (calcyclin) S100 calcium binding protein A6 S100 calcium-binding protein A6 S100A6 S10A6_HUMAN |
Images
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Fig1:
Immunocytochemistry analysis of C6 cells labeling S100 alpha 6 with Rabbit anti-S100 alpha 6 antibody (HA724357F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-S100 alpha 6 antibody (HA724357F, FITC, green) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling S100 alpha 6 with Rabbit anti-S100 alpha 6 antibody (HA724357F) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA724357F, FITC, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.