XBP1 Recombinant Rabbit Monoclonal Antibody [PSH23-23]
cat.: HA724358
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH23-23 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29/40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human XBP1s aa 327-376. |
| Positive control: | Huh7 (Human liver cancer cells) cell lysate, Huh7 (Human liver cancer cells) treated with 300nM Thapsigargin for 18 hours cell lysate, C6 (Rat glioma cells) cell lysate, C6 (Rat glioma cells) treated with 300nM Thapsigargin for 18 hours cell lysate. |
| Subcellular location: | Nucleus, Cytoplasm, Endoplasmic reticulum membrane, Endoplasmic reticulum membrane, Membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P17861 Human | O35426 Mouse | Q9R1S4 Rat |
| Alternative names: | Tax responsive element binding protein 5 Tax-responsive element-binding protein 5 TREB5 X box binding protein 1 X box binding protein 2 X-box-binding protein 1 XBP 1 XBP-1 XBP1 XBP1_HUMAN XBP2 |
Images
|
Fig1:
Western blot analysis of XBP1 on different lysates with Rabbit anti-XBP1 antibody (HA724358) at 1/5,000 dilution. Lane 1: Huh7 (Human liver cancer cells) cell lysate Lane 2: Huh7 (Human liver cancer cells) treated with 300nM Thapsigargin for 18 hours cell lysate Lane 3: C6 (Rat glioma cells) cell lysate Lane 4: C6 (Rat glioma cells) treated with 300nM Thapsigargin for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 24 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724358, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 29, 40 kDa Observed band size: 29, 56 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.