Histone H3 (citrulline R2) Recombinant Rabbit Monoclonal Antibody [PSH23-25]
cat.: HA724360
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH23-25 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 15 kDa |
| Isotype: | IgG |
| Positive control: | 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB IP |
1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: P68431 Human |
| Alternative names: | H3FA HIST1H3A H3C2 H3FL HIST1H3B H3C3 H3FC HIST1H3C H3C4 H3FB HIST1H3D H3C6 H3FD HIST1H3E H3C7 H3FI HIST1H3F H3C8 H3FH HIST1H3G H3C10 H3FK HIST1H3H H3C11 H3FF HIST1H3I H3C12 H3FJ HIST1H3J HIST1H3C H3FC H3C1 Histone H3.1 Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3/f Histone H3/h Histone H3/i Histone H3/j Histone H3/k Histone H3/l |
Images
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Fig1:
Western blot analysis of Histone H3 (citrulline R2) on different lysates with Rabbit anti-Histone H3 (citrulline R2) antibody (HA724360) at 1/2,000 dilution.
Lane 1: 293T (Human embryonic kidney cells) cells transfected with PADI4 Lane 2: 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724360, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 15 kDa Observed band size: 15 kDa |
|
Fig2:
Immunoprecipitation (IP)
Histone H3 (citrulline R2) was immunoprecipitated in 0.2 mg 293T (Human embryonic kidney cells) cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate with HA724360 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724360 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate (input) Lane 2: HA724360 IP in 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate Lane 3: Rabbit IgG instead of HA724360 in 293T cells transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 2 hours cell lysate Exposure time: 2 minutes Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724360, 1/1,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 15 kDa Observed band size: 15 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.