Mouse/Rat MMP-3 Recombinant Rabbit Monoclonal Antibody [PSH23-35]
cat.: HA724366
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH23-35 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse MMP-3 aa 18-477 (HA211280). |
| Positive control: | Recombinant Mouse MMP-3 protein (HA211280). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-34] to Mouse/Rat MMP-3 (Capture) (HA724365) and Recombinant Mouse MMP-3 protein (HA211280) as the standard. The reference range value is 19.5-5,000 pg/mL. |
| Uniprot #: | SwissProt: P28862 Mouse | P03957 Rat |
| Alternative names: | CHDS6 Matrix metalloproteinase 3 Matrix metalloproteinase-3 MGC126102 MGC126103 MGC126104 MMP 3 MMP-3 MMP3 Proteoglycanase SL-1 SL1 STMY STMY1 STR1 Stromelysin 1 Stromelysin-1 Transin 1 Transin-1 |
Images
|
Fig1:
Sandwich ELISA analysis of Mouse/Rat MMP-3 matched pair antibodies Capture: HA724365, Mouse/Rat MMP-3 Rabbit mAb [PSH23-34] Detector: HA724366, Mouse/Rat MMP-3 Rabbit mAb [PSH23-35] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724365) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse MMP-3 protein (HA211280) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA724366, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native MMP3 in Mouse and Rat serum. Capture: HA724365, Mouse/Rat MMP-3 Rabbit mAb [PSH23-34] Detector: HA724366, Mouse/Rat MMP-3 Rabbit mAb [PSH23-35] Interpolated concentration of native MMP3 was measured in duplicate at different sample concentrations and interpolated from the MMP3 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean MMP3 concentration was determined to be 74,092 pg/ml in Mouse serum and 11,892 pg/ml in Rat serum. |
|
Fig3:
Interpolated concentrations of spiked MMP3 in cell culture media samples. Capture: HA724365, Mouse/Rat MMP-3 Rabbit mAb [PSH23-34] Detector: HA724366, Mouse/Rat MMP-3 Rabbit mAb [PSH23-35] The concentrations of MMP3 were measured in duplicates, interpolated from the MMP3 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.