Human Thrombomodulin Recombinant Rabbit Monoclonal Antibody [PSH23-37]
cat.: HA724368
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH23-37 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Thrombomodulin aa 239-515 (HA211324). |
| Positive control: | Recombinant Human Thrombomodulin protein (HA211324). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-36] to Human Thrombomodulin (Capture) (HA724367) and Recombinant Human Thrombomodulin protein (HA211324) as the standard. The reference range value is 39.1-10,000 pg/mL. |
| Uniprot #: | SwissProt: P07204 Human |
| Alternative names: | AHUS 6 AHUS6 BDCA 3 BDCA3 CD 141 CD141 CD141 antigen Fetomodulin Thbd THPH12 THRM Thrombomodulin TM TRBM_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of Human Thrombomodulin matched pair antibodies Capture: HA724367, Human Thrombomodulin Rabbit mAb [PSH23-36] Detector: HA724368, Human Thrombomodulin Rabbit mAb [PSH23-37] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724367) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Thrombomodulin protein (HA211324) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724368, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Thrombomodulin in human urine and serum. Capture: HA724367, Human Thrombomodulin Rabbit mAb [PSH23-36] Detector: HA724368, Human Thrombomodulin Rabbit mAb [PSH23-37] Interpolated concentration of native Thrombomodulin was measured in duplicate at different sample concentrations and interpolated from the Thrombomodulin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Thrombomodulin concentration was determined to be 14,736 pg/ml in human urine and 5,813 pg/ml in human serum. |
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Fig3:
Interpolated concentrations of spiked Thrombomodulin in cell culture media samples. Capture: HA724367, Human Thrombomodulin Rabbit mAb [PSH23-36] Detector: HA724368, Human Thrombomodulin Rabbit mAb [PSH23-37] The concentrations of Thrombomodulin were measured in duplicates, interpolated from the Thrombomodulin standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.