Biotin Conjugated Human CCL24/Eotaxin 2 Recombinant Rabbit Monoclonal Antibody [PSH23-39]
cat.: HA724370B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH23-39 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCL24/Eotaxin 2 aa 27-119 (HA211257). |
| Positive control: | Recombinant Human CCL24/Eotaxin 2 protein (HA211257). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-38] to Human CCL24/Eotaxin 2 (Capture) (HA724369) and Recombinant Human CCL24/Eotaxin 2 protein (HA211257) as the standard. The reference range value is 7.8-2,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O00175 Human |
| Alternative names: | C C motif chemokine 24 C-C motif chemokine 24 CCL24 CCL24_HUMAN Chemokine CC Motif Ligand 24 CK beta 6 CK-beta-6 Ckb6 Eosinophil chemotactic protein 2 Eotaxin-2 MPIF 2 MPIF-2 MPIF2 Myeloid progenitor inhibitory factor 2 SCYA24 Small inducible cytokine A24 Small inducible cytokine subfamily A (Cys-Cys) member 24 Small-inducible cytokine A24 |
Images
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Fig1:
Sandwich ELISA analysis of Human CCL24/Eotaxin 2 matched pair antibodies Capture: HA724369, Human CCL24/Eotaxin 2 Rabbit mAb [PSH23-38] Detector: HA724370, Human CCL24/Eotaxin 2 Rabbit mAb [PSH23-39] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724369) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.2% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL24/Eotaxin 2 protein (HA211257) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA724370, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CCL24/Eotaxin 2 in human serum samples. Capture: HA724369, Human CCL24/Eotaxin 2 Rabbit mAb [PSH23-38] Detector: HA724370, Human CCL24/Eotaxin 2 Rabbit mAb [PSH23-39] The concentrations of CCL24/Eotaxin 2 were measured in duplicates, interpolated from the CCL24/Eotaxin 2 standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL24/Eotaxin 2 concentration was determined to be 916.6 pg/ml in human serum. |
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Fig3:
Interpolated concentrations of spiked CCL24/Eotaxin 2 in human cell culture media samples. Capture: HA724369, Human CCL24/Eotaxin 2 Rabbit mAb [PSH23-38] Detector: HA724370, Human CCL24/Eotaxin 2 Rabbit mAb [PSH23-39] The concentrations of CCL24/Eotaxin 2 were measured in duplicates, interpolated from the CCL24/Eotaxin 2 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.