Human Sclerostin Recombinant Rabbit Monoclonal Antibody [PSH23-43]
cat.: HA724374
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH23-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Sclerostin aa 24-213 (HA211397). |
| Positive control: | Recombinant Human Sclerostin protein (HA211397). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-42] to Human Sclerostin (Capture) (HA724373) and Recombinant Human Sclerostin protein (HA211397) as the standard. The reference range value is 15.6-4,000 pg/mL. |
| Uniprot #: | SwissProt: Q9BQB4 Human |
| Alternative names: | BEER CDD Cortical hyperostosis with syndactyly Sclerosteosis Sclerostin Sost SOST_HUMAN SOST1 UNQ2976/PRO7455/PRO7476 VBCH |
Images
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Fig1:
Sandwich ELISA analysis of Human Sclerostin matched pair antibodies Capture: HA724373, Human Sclerostin Rabbit mAb [PSH23-42] Detector: HA724374, Human Sclerostin Rabbit mAb [PSH23-43] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724373) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Sclerostin protein (HA211397) protein starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA724374, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Sclerostin in human samples. Capture: HA724373, Human Sclerostin Rabbit mAb [PSH23-42] Detector: HA724374, Human Sclerostin Rabbit mAb [PSH23-43] Interpolated concentration of native Sclerostin was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Sclerostin concentration was determined to be 341 pg/mL in human serum. There was no detectable signal in HepG2 cell culture supernatant. |
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Fig3:
Interpolated concentrations of spiked Sclerostin in cell culture media samples. Capture: HA724373, Human Sclerostin Rabbit mAb [PSH23-42] Detector: HA724374, Human Sclerostin Rabbit mAb [PSH23-43] The concentrations of Sclerostin were measured in duplicates, interpolated from the Sclerostin standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.