Biotin Conjugated Human Ataxin 3 Recombinant Rabbit Monoclonal Antibody [PSH23-45]
cat.: HA724376B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH23-45 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Ataxin 3 aa 1-361 (HA211529). |
| Positive control: | Recombinant Human Ataxin 3 protein (HA211529). |
| Subcellular location: | Nucleus matrix, Nucleus, Lysosome membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-44] to Human Ataxin 3 (Capture) (HA724375) and Recombinant Human Ataxin 3 protein (HA211529) as the standard. The reference range value is 39.1-10,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P54252 Human |
| Alternative names: | AT3 Ataxin 3 ataxin 3 variant h ataxin 3 variant m ataxin 3 variant ref Ataxin-3 ATX3 ATX3_HUMAN ATXN3 EC 3.4.22. JOS Josephin Machado Joseph disease (spinocerebellar ataxia 3 olivopontocerebellar ataxia 3 autosomal dominant ataxin 3) Machado Joseph disease Machado Joseph disease protein 1 Machado-Joseph disease protein 1 Machado-Joseph disease protein 1 homolog MJD MJD gene MJD1 Olivopontocerebellar ataxia 3 OTTHUMP00000221583 OTTHUMP00000221585 OTTHUMP00000221586 OTTHUMP00000221587 OTTHUMP00000231995 OTTHUMP00000231997 Rsca3 SCA3 SCA3 gene Spinocerebellar ataxia type 3 protein |
Images
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Fig1:
Sandwich ELISA analysis of Human Ataxin 3 matched pair antibodies Capture: HA724375, Human Ataxin 3 Rabbit mAb [PSH23-44] Detector: HA724376, Human Ataxin 3 Rabbit mAb [PSH23-45] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724375) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.2% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Ataxin 3 protein (HA211529) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724376, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Ataxin 3 in HeLa cell extract samples based on a 1,000 µg/ml extract load. Capture: HA724375, Human Ataxin 3 Rabbit mAb [PSH23-44] Detector: HA724376, Human Ataxin 3 Rabbit mAb [PSH23-45] The concentrations of Ataxin 3 were measured in duplicates, interpolated from the Ataxin 3 standard curve and corrected for sample dilution. Undiluted samples are HeLa cell extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ataxin 3 concentration was determined to be 6,568 pg/ml in HeLa cell extract. |
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Fig3:
Interpolated concentrations of spiked Ataxin 3 in human cell culture media samples. Capture: HA724375, Human Ataxin 3 Rabbit mAb [PSH23-44] Detector: HA724376, Human Ataxin 3 Rabbit mAb [PSH23-45] The concentrations of Ataxin 3 were measured in duplicates, interpolated from the Ataxin 3 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.