STX17 Recombinant Rabbit Monoclonal Antibody [PSH23-50]
cat.: HA724381
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH23-50 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human STX17 aa 1-250. |
| Positive control: | HeLa (Human cervical adenocarcinoma cells) cell lysate, 293T (Human embryonic kidney cells) cell lysate, Daudi (Human Burkitt's lymphoma cells) cell lysate, K-562 (Human chronic myelogenous leukemia cells) cell lysate, A431 (Human epidermoid carcinoma skin squamous cells) cell lysate, C2C12 (Mouse myoblasts) cell lysate, L6 (Rat skeletal muscle myoblasts) cell lysate, COS-1 (African green monkey kidney fibroblasts) cell lysate. |
| Subcellular location: | Endoplasmic reticulum membrane, Smooth endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Cytoplasmic vesicle, autophagosome membrane, COPII-coated vesicle membrane, Cytoplasm, cytosol, Mitochondrion membrane, Autolysosome membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:10,000-1:25,000 1:200 1:1,000 |
| Uniprot #: | SwissProt: P56962 Human | Q9D0I4 Mouse | Q9Z158 Rat |
| Alternative names: | Syntaxin-17 STX17 |
Images
|
Fig1:
Western blot analysis of STX17 on different lysates with Rabbit anti-STX17 antibody (HA724381) at 1/10,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 2: 293T (Human embryonic kidney cells) cell lysate Lane 3: Daudi (Human Burkitt's lymphoma cells) cell lysate Lane 4: K-562 (Human chronic myelogenous leukemia cells) cell lysate Lane 5: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate Lane 6: C2C12 (Mouse myoblasts) cell lysate Lane 7: L6 (Rat skeletal muscle myoblasts) cell lysate Lane 8: COS-1 (African green monkey kidney fibroblasts) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 14 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724381, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 33 kDa Observed band size: 35 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724381, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724381, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724381, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Liver Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724381, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Prostate Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724381, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig7:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Prostate Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724381, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig8:
Application: Flow Cytometry (Intra)
Species: Mouse Sample: C2C12 (Mouse myoblasts) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724381 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
|
Fig9:
Application: Flow Cytometry (Intra)
Species: Rat Sample: L6 (Rat skeletal muscle myoblasts) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724381 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.