STEAP1 Recombinant Rabbit Monoclonal Antibody [PSH23-51]
cat.: HA724382
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH23-51 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human aa 1-339. |
| Positive control: | LNCaP (Human prostate cancer cells) cell lysate, Caco-2 (Human colorectal adenocarcinoma cells) cell lysate, LoVo (Human colon cancer cells) cell lysate, U-87 MG (Human glioblastoma cells) cell lysate, COLO205 (Human colon adenocarcinoma cells) cell lysate. |
| Subcellular location: | Endosome membrane, Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:50,000 1:500 1:3,000 1:1,000 |
| Uniprot #: | SwissProt: Q9UHE8 Human |
| Alternative names: | 2410007B19Rik EC 1.16.1.- Metalloreductase STEAP1 MGC19484 PRSS 24 PRSS24 Six transmembrane epithelial antigen of prostate Six transmembrane epithelial antigen of the prostate 1 Six transmembrane epithelial antigen of the prostate Six-transmembrane epithelial antigen of prostate 1 STEA1_HUMAN STEAP 1 Steap STEAP1 |
Images
|
Fig1:
Western blot analysis of STEAP1 on different lysates with Rabbit anti-STEAP1 antibody (HA724382) at 1/50,000 dilution.
Lane 1: LNCaP (Human prostate cancer cells) cell lysate Lane 2: MCF7 (Human breast cancer cells) cell lysate Lane 3: Caco-2 (Human colorectal adenocarcinoma cells) cell lysate Lane 4: LoVo (Human colon cancer cells) cell lysate Lane 5: U-87 MG (Human glioblastoma cells) cell lysate Lane 6: COLO205 (Human colon adenocarcinoma cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 15 seconds; ECL: K1801 MCF7 is a negative control (PMID: 16622681). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724382, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 40 kDa Observed band size: 35 kDa |
|
Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: LNCaP (Human prostate cancer cells)/MCF7 (Human breast cancer cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724382, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). Negative control: MCF7 (PMID: 16622681). |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Prostate Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724382, 1/3,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Prostate carcinoma Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724382, 1/3,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Cerebrum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724382, 1/3,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Low expression : Human cerebrum (PMID: 10588738) |
|
Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724382, 1/3,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Low expression : Human colon (PMID: 10588738) |
|
Fig7:
Application: Flow Cytometry (Intra) Species: Human Sample: LNCaP (Human prostate cancer cells)/MCF7 (Human breast cancer cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724382 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. Negative control: MCF7(PMID: 16622681). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.