Biotin Conjugated Human FGF23 Recombinant Rabbit Monoclonal Antibody [PSH23-71]
cat.: HA724394B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH23-71 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FGF23 aa 25-251 (HA211378). |
| Positive control: | Recombinant Human FGF23 protein (HA211378). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-70] to Human FGF23 (Capture) (HA724393) and Recombinant Human FGF23 protein (HA211378) as the standard. The reference range value is 39.1-10,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9GZV9 Human |
| Alternative names: | ADHR FGF-23 Fgf23 FGF23_HUMAN FGFN Fibroblast growth factor 23 Fibroblast growth factor 23 C-terminal peptide Fibroblast growth factor 23 precursor HPDR2 HYPF Phosphatonin PHPTC Tumor derived hypophosphatemia inducing factor Tumor-derived hypophosphatemia-inducing factor |
Images
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Fig1:
Sandwich ELISA analysis of Human FGF23 matched pair antibodies Capture: HA724393, Human FGF23 Rabbit mAb [PSH23-70] Detector: HA724394, Human FGF23 Rabbit mAb [PSH23-71] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724393) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human FGF23 protein (HA211378) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724394, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of spiked FGF23 in human cell culture media samples. Capture: HA724393, Human FGF23 Rabbit mAb [PSH23-70] Detector: HA724394, Human FGF23 Rabbit mAb [PSH23-71] The concentrations of FGF23 were measured in duplicates, interpolated from the FGF23 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.