Cardiac Troponin I Recombinant Rabbit Monoclonal Antibody [PSH23-75]
cat.: HA724398
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH23-75 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Positive control: | Mouse heart tissue lysate, Rat heart tissue lysate. |
| Subcellular location: | Cytosol. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P48787 Mouse | P23693 Rat |
| Alternative names: | cardiac muscle Cardiac troponin I cardiomyopathy, dilated 2A (autosomal recessive) Cardiomyopathy, familial hypertrophic, 7, included CMD1FF CMD2A CMH7 cTnI Familial hypertrophic cardiomyopathy 7 MGC116817 RCM1 Tn1 Tni TNN I3 TNNC 1 TNNC1 TNNI3 Troponin I Troponin I cardiac Troponin I cardiac muscle Troponin I cardiac muscle isoform Troponin I type 3 cardiac troponin I, cardiac 3 TroponinI Ttroponin I type 3 (cardiac) |
Images
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Fig1:
Western blot analysis of Cardiac Troponin I on different lysates with Rabbit anti-Cardiac Troponin I antibody (HA724398) at 1/2,000 dilution.
Lane 1: Mouse heart tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Lane 3: Rat heart tissue lysate Lane 4: Rat skeletal muscle tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 49 seconds; ECL: K1801 Negative expression of Cardiac Troponin I protein in skeletal muscle is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724398, 1/2,000 in 5% NFDM/TBST, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 24 kDa Observed band size: 25 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.