Phospho-Syk (Y526) Recombinant Rabbit Monoclonal Antibody [PSH23-78]
cat.: HA724399
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH23-78 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 72 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Tyr526 of Human Syk. |
| Positive control: | Ramos treated with 12ug/mL human IgM for 10min cell lysate. |
| Subcellular location: | Cell membrane, Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IP |
1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: P43405 Human |
| Alternative names: | EC 2.7.10.2 kinase Syk KSYK KSYK_HUMAN p72-Syk p72syk Spleen tyrosine kinase Syk Tyrosine protein kinase SYK Tyrosine-protein kinase SYK |
Images
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Fig1:
Western blot analysis of Phospho-Syk (Y526) on different lysates with Rabbit anti-Phospho-Syk (Y526) antibody (HA724399) at 1/5,000 dilution. Lane 1: Ramos (Human Burkitt's lymphoma cells) cell lysate Lane 2: Ramos treated with 12ug/mL human IgM for 10min cell lysate Lane 3: Ramos treated with 12ug/mL human IgM for 10min cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Exposure time: 42 seconds; ECL: K1801 Total Syk protein was detected with another antibody (abcam, ab40781) Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724399, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 72 kDa Observed band size: 72 kDa |
|
Fig2:
Immunoprecipitation (IP) Phospho-Syk (Y526) was immunoprecipitated in 0.2 mg Ramos (Human Burkitt's lymphoma cells) treated with 12ug/mL human IgM for 10min cell lysate with HA724399 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724399 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Ramos (Human Burkitt's lymphoma cells) treated with 12ug/mL human IgM for 10min cell lysate (input) Lane 2: HA724399 IP in Ramos (Human Burkitt's lymphoma cells) treated with 12ug/mL human IgM for 10min cell lysate Lane 3: Rabbit IgG instead of HA724399 in Ramos (Human Burkitt's lymphoma cells) treated with 12ug/mL human IgM for 10min cell lysate Exposure time: 30 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724399, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 72 kDa Observed band size: 72 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.