CEP55 Recombinant Rabbit Monoclonal Antibody [PSH23-80]
cat.: HA724401
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH23-80 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CEP55 aa 1-464. |
| Positive control: | LNCaP (Human prostate cancer cells) cell lysate, HeLa (Human cervical adenocarcinoma cells) cell lysate, MDA-MB-231 (Human breast cancer cells) cell lysate, SK-MEL-28 (Human malignant melanoma cells) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole, Cleavage furrow, Midbody, Midbody ring. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000-1:20,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q53EZ4 Human | Q8BT07 Mouse | Q4V7C8 Rat |
| Alternative names: | C10orf3 cancer/testis antigen 111 Centrosomal protein 55kDa Centrosomal protein of 55 kDa CEP 55 Cep55 CEP55_HUMAN CT111 FLJ10540 Up regulated in colon cancer 6 Up-regulated in colon cancer 6 URCC 6 URCC6 |
Images
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Fig1:
Western blot analysis of CEP55 on different lysates with Rabbit anti-CEP55 antibody (HA724401) at 1/20,000 dilution. Lane 1: LNCaP (Human prostate cancer cells) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 3: MDA-MB-231 (Human breast cancer cells) cell lysate Lane 4: SK-MEL-28 (Human malignant melanoma cells) cell lysate Lane 5: NIH/3T3 (Mouse fibroblasts) cell lysate Lane 6: PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724401, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 54 kDa Observed band size: 54 kDa |
|
Fig2:
Western blot analysis of CEP55 on different lysates with Rabbit anti-CEP55 antibody (HA724401) at 1/5,000 dilution.
Lane 1: Mouse testis tissue lysate Lane 2: Rat testis tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 12 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724401, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 54 kDa Observed band size: 54 kDa |
|
Fig3:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: MDA-MB-231 (Human breast cancer cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724401, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). |
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Fig4:
Application: Immunocytochemistry (IF-cell)
Species: Mouse Sample: NIH/3T3 (Mouse fibroblasts) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724401, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). |
|
Fig5:
Application: Flow Cytometry (Intra)
Species: Human Sample: MDA-MB-231 (Human breast cancer cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724401 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
|
Fig6:
Application: Flow Cytometry (Intra)
Species: Mouse Sample: NIH/3T3 (Mouse fibroblasts) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724401 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.