CD27 Recombinant Rabbit Monoclonal Antibody [PSH23-81]
cat.: HA724402
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH23-81 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse CD27 aa 1-182. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P41272 Mouse |
| Alternative names: | CD 27 CD27 CD27 antigen CD27 molecule CD27L receptor LPFS2 MGC20393 OTTHUMP00000238557 S152 T cell activation antigen CD27 T cell antivation antigen S152 T-cell activation antigen CD27 T14 TNFRSF 7 TNFRSF7 TNFSF7 Tp 55 Tp55 Tumor necrosis factor receptor superfamily member 7 |
Images
|
Fig1:
Western blot analysis of CD27 on different lysates with Rabbit anti-CD27 antibody (HA724402) at 1/5,000 dilution.
Lane 1: Mouse thymus tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Mouse liver tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724402, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 28 kDa Observed band size: 50-55/28 kDa The molecular weight is consistent with the article (PMID: 38354704, PMID: 38609999). Low expression of CD27 protein in liver is consistent with the predicted expression pattern. |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724402, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Thymus Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724402, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Immunoprecipitation (IP)
CD27 was immunoprecipitated in 0.2 mg mouse thymus tissue lysate with HA724402 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724402 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse thymus tissue lysate (input) Lane 2: HA724402 IP in mouse thymus tissue lysate Lane 3: Rabbit IgG instead of HA724402 in mouse thymus tissue lysate Exposure time: 20seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724402, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 28 kDa Observed band size: 50-55/28 kDa |
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Fig5:
Application: Flow Cytometry
Species: Mouse Sample: C57BL/6 Mouse PBMC Blocking: 1% BSA + 10% normal goat serum + CD16/CD32 (HA601571), 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: mouse CD3-APC (HA720201F) and CD27-PE (HA724402-PE, Right) or Rabbit IgG Isotype Control-PE (HA720192F, left), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.