PCK1 Recombinant Rabbit Monoclonal Antibody [PSH23-82]
cat.: HA724404
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH23-82 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PCK1 aa 573-622. |
| Positive control: | Mouse liver tissue lysate, Mouse kidney tissue lysate, Mouse brown adipose tissue lysate, Rat liver tissue lysate, Rat kidney tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, Endoplasmic reticulum. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q9Z2V4 Mouse | P07379 Rat |
| Alternative names: | cytosolic [GTP] GTP PCK1 PEP carboxykinase PEPCK-C PEPCK1 PEPCKC Phosphoenolpyruvate carboxykinase 1 (soluble) Phosphoenolpyruvate carboxykinase 1 Phosphoenolpyruvate carboxykinase Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Phosphoenolpyruvate carboxykinase, cytosolic Phosphoenolpyruvate carboxylase Phosphopyruvate carboxylase |
Images
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Fig1:
Western blot analysis of PCK1 on different lysates with Rabbit anti-PCK1 antibody (HA724404) at 1/5,000 dilution.
Lane 1: Mouse liver tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Mouse kidney tissue lysate Lane 4: Mouse brown adipose tissue lysate Lane 5: Rat liver tissue lysate Lane 6: Rat spleen tissue lysate Lane 7: Rat kidney tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 40 seconds; ECL: K1801 Low expression: spleen (PMID: 36918564) Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724404, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 69 kDa Observed band size: 69 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.