Dopamine beta Hydroxylase Recombinant Rabbit Monoclonal Antibody [PSH23-88]
cat.: HA724406
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH23-88 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Dopamine beta Hydroxylase aa 1-622. |
| Positive control: | Mouse adrenal gland tissue lysate, Rat adrenal gland tissue lysate. |
| Subcellular location: | Cytoplasmic vesicle, secretory vesicle membrane, secretory vesicle, chromaffin granule membrane. |
| Recommended Dilutions:
WB IHC-Fr IHC-P IP |
1:5,000 1:500 1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q64237 Mouse | Q05754 Rat |
| Alternative names: | dbh DBM Dopamine beta hydroxylase Dopamine beta monooxygenase Dopamine beta-hydroxylase (dopamine beta-monooxygenase) Dopamine beta-monooxygenase OTTHUMP00000022501 Soluble dopamine beta-hydroxylase |
Images
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Fig1:
Western blot analysis of Dopamine beta Hydroxylase on different lysates with Rabbit anti-Dopamine beta Hydroxylase antibody (HA724406) at 1/5,000 dilution.
Lane 1: Mouse adrenal gland tissue lysate Lane 2: Mouse spinal cord tissue lysate Lane 3: Mouse testis tissue lysate Lane 4: Rat adrenal gland tissue lysate Lane 5: Rat spinal cord tissue lysate Lane 6: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Spinal cord is a negative control (PMID: 8182421). Low expression of Dopamine beta Hydroxylase protein in testis is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724406, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 70 kDa Observed band size: 75-80 kDa |
|
Fig2:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Adrenal gland Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724406, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig3:
Application: Immunofluorescence (IHC-Fr) Species: Rat Tissue: Adrenal gland Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724406, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Adrenal gland Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724406, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Brainstem Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724406, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Adrenal gland Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724406, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig7:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Brainstem Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724406, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig8:
Immunoprecipitation (IP)
Dopamine beta Hydroxylase was immunoprecipitated in 0.2 mg mouse adrenal gland tissue lysate with HA724406 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724406 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse adrenal gland tissue lysate (input) Lane 2: HA724406 IP in mouse adrenal gland tissue lysate Lane 3: Rabbit IgG instead of HA724406 in mouse adrenal gland tissue lysate Exposure time: 1 minute 55 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724406, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 70 kDa Observed band size: 75-80 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.