Gemin 1 Recombinant Rabbit Monoclonal Antibody [PSH23-89]
cat.: HA724407
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH23-89 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Gemin 1 aa 1-236. |
| Positive control: | HeLa (Human cervical adenocarcinoma cells) cell lysate, Hep G2 (Human liver cancer cells) cell lysate, 293T (Human embryonic kidney cells) cell lysate, U-87 MG (Human glioblastoma cells) cell lysate, SH-SY5Y (Human neuroblastoma cells) cell lysate, K-562 (Human chronic myelogenous leukemia cells) cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC IP |
1:5,000 1:100 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q16637 Human |
| Alternative names: | BCD541 Component of gems 1 Gemin 1 Gemin-1 OTTHUMP00000125198 OTTHUMP00000223567 OTTHUMP00000223568 OTTHUMP00000224066 OTTHUMP00000226924 SMA 1 SMA 2 SMA 3 SMA 4 SMA SMA@ SMA1 SMA2 SMA3 SMA4 SMN SMN_HUMAN SMN1 SMN2 SMNT Survival motor neuron protein Survival of motor neuron 1, telomeric T-BCD541 |
Images
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Fig1:
Western blot analysis of Gemin 1 on different lysates with Rabbit anti-Gemin 1 antibody (HA724407) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 2: Hep G2 (Human liver cancer cells) cell lysate Lane 3: 293T (Human embryonic kidney cells) cell lysate Lane 4: U-87 MG (Human glioblastoma cells) cell lysate Lane 5: SH-SY5Y (Human neuroblastoma cells) cell lysate Lane 6: K-562 (Human chronic myelogenous leukemia cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724407, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 32 kDa Observed band size: 35-38 kDa |
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Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: HeLa (Human cervix adenocarcinoma epithelial cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724407, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). |
|
Fig3:
Application: Flow Cytometry (Intra)
Species: Human Sample: HeLa (Human cervix adenocarcinoma epithelial cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724407 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
|
Fig4:
Immunoprecipitation (IP)
Gemin 1 was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with HA724407 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724407 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA724407 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA724407 in HeLa cell lysate Exposure time: 11 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724407, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 32 kDa Observed band size: 35-38 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.