GATA4 Recombinant Rabbit Monoclonal Antibody [PSH23-98]
cat.: HA724409
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC(Intra) |
| Clonality: | Monoclonal |
| Clone number: | PSH23-98 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GATA4 aa 1-442. |
| Positive control: | Hep G2 (Human liver cancer cells) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, Mouse ovary tissue lysate, Rat testis tissue lysate, Rat ovary tissue lysate. |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB IF-Cell IHC-P FC(Intra) |
1:2,000-1:5,000 1:100 1:200 1:1,000 |
| Uniprot #: | SwissProt: P43694 Human | Q08369 Mouse | P46152 Rat |
| Alternative names: | ASD2 GATA 4 GATA binding protein 4 GATA-binding factor 4 gata4 GATA4_HUMAN MGC126629 Transcription factor GATA 4 Transcription factor GATA-4 Transcription factor GATA4 VSD1 |
Images
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Fig1:
Western blot analysis of GATA4 on different lysates with Rabbit anti-GATA4 antibody (HA724409) at 1/5,000 dilution.
Lane 1: Hep G2 (Human liver cancer cells) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cells) cell lysate Lane 3: NIH/3T3 (Mouse fibroblasts) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 2 minutes; ECL: K1802 HeLa is a negative control (PMID: 24696446). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724409, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45 kDa Observed band size: 50 kDa |
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Fig2:
Western blot analysis of GATA4 on different lysates with Rabbit anti-GATA4 antibody (HA724409) at 1/5,000 dilution.
Lane 1: Mouse ovary tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Skeletal muscle tissue is a negative control (PMID: 22808244). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724409, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45 kDa Observed band size: 50 kDa |
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Fig3:
Western blot analysis of GATA4 on different lysates with Rabbit anti-GATA4 antibody (HA724409) at 1/2,000 dilution.
Lane 1: Rat testis tissue lysate Lane 2: Rat ovary tissue lysate Lane 3: Rat skeletal muscle tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 1 minute; ECL: K1801 Skeletal muscle tissue is a negative control (PMID: 22808244). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724409, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45 kDa Observed band size: 50 kDa |
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Fig4:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: Hep G2 (Human liver cancer cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724409, 1/100, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Testis Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724409, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig6:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Skeletal muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724409, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Skeletal muscle tissue is a negative control (PMID：22808244). |
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Fig7:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Testis Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724409, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig8:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Skeletal muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724409, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Skeletal muscle tissue is a negative control (PMID：22808244). |
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Fig9:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Testis Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724409, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig10:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Skeletal muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724409, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Skeletal muscle tissue is a negative control (PMID：22808244). |
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Fig11:
Application: Flow Cytometry (Intra)
Species: Human Sample: Hep G2 (Human liver cancer cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724409 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
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Fig12:
Application: Flow Cytometry (Intra) Species: Mouse Sample: NIH/3T3 (Mouse fibroblasts) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724409 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.