Biotin Conjugated Human CCL1 Recombinant Rabbit Monoclonal Antibody [PSH24-05]
cat.: HA724417B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH24-05 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCL1 aa 24-96 (HA211081). |
| Positive control: | Recombinant Human CCL1 protein (HA211081). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-04] to Human CCL1 (Capture) (HA724416) and Recombinant Human CCL1 protein (HA211081) as the standard. The reference range value is 5.9-1,500 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P22362 Human |
| Alternative names: | C-C motif chemokine 1 Small-inducible cytokine A1 T lymphocyte-secreted protein I-309 CCL1 SCYA1 |
Images
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Fig1:
Sandwich ELISA analysis of Human CCL1 matched pair antibodies Capture: HA724416, Human CCL1 Rabbit mAb [PSH24-04] Detector: HA724417, Human CCL1 Rabbit mAb [PSH24-05] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724416) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CCL1 protein (HA211081) starting from 1,500 pg/ml to 0 pg/ml and detect antibody (HA724417, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CCL1 in human Jurkat supernatant treated or untreated with PHA and TPA for 24 hours. Capture: HA724416, Human CCL1 Rabbit mAb [PSH24-04] Detector: HA724417, Human CCL1 Rabbit mAb [PSH24-05] Interpolated concentration of native CCL1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CCL1 concentration was determined to be 58 pg/mL in neat Jurkat treated supernatant and 5 pg/mL in untreated Jurkat supernatant. |
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Fig3:
Interpolated concentrations of spiked CCL1 in cell culture media samples. Capture: HA724416, Human CCL1 Rabbit mAb [PSH24-04] Detector: HA724417, Human CCL1 Rabbit mAb [PSH24-05] The concentrations of CCL1 were measured in duplicates, interpolated from the CCL1 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.