Human Gemin 1 Recombinant Rabbit Monoclonal Antibody [PSH24-07]
cat.: HA724419
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH24-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Gemin 1 aa 11-216 (HA211276). |
| Positive control: | Recombinant Human Gemin 1 protein (HA211276). |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-06] to Human Gemin 1 (Capture) (HA724418) and Recombinant Human Gemin 1 protein (HA211276) as the standard. The reference range value is 31.3-4,000 pg/mL. |
| Uniprot #: | SwissProt: Q16637 Human |
| Alternative names: | BCD541 Component of gems 1 Gemin 1 Gemin-1 OTTHUMP00000125198 OTTHUMP00000223567 OTTHUMP00000223568 OTTHUMP00000224066 OTTHUMP00000226924 SMA 1 SMA 2 SMA 3 SMA 4 SMA SMA@ SMA1 SMA2 SMA3 SMA4 SMN SMN_HUMAN SMN1 SMN2 SMNT Survival motor neuron protein Survival of motor neuron 1, telomeric T-BCD541 |
Images
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Fig1:
Sandwich ELISA analysis of Human Gemin 1 matched pair antibodies Capture: HA724418, Human Gemin 1 Rabbit mAb [PSH24-06] Detector: HA724419, Human Gemin 1 Rabbit mAb [PSH24-07] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724418) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Gemin 1 protein (HA211276) starting from 3,000 pg/ml to 0 pg/ml and detect antibody (HA724419, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Gemin 1 in HeLa and SH-SY5Y cell extract samples based on a 1,000 µg/ml extract load. Capture: HA724418, Human Gemin 1 Rabbit mAb [PSH24-06] Detector: HA724419, Human Gemin 1 Rabbit mAb [PSH24-07] The concentrations of Gemin 1 were measured in duplicates, interpolated from the Gemin 1 standard curve and corrected for sample dilution. Undiluted samples are HeLa cell extract 10% and SH-SY5Y cell extract 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Gemin 1 concentration was determined to be 7,780 pg/ml in HeLa cell extract and 15,999 pg/ml SH-SY5Y cell extract. |
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Fig3:
Interpolated concentrations of spiked Gemin 1 in human cell culture media samples. Capture: HA724418, Human Gemin 1 Rabbit mAb [PSH24-06] Detector: HA724419, Human Gemin 1 Rabbit mAb [PSH24-07] The concentrations of Gemin 1 were measured in duplicates, interpolated from the Gemin 1 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.