Mouse CXCL10 / IP-10 Recombinant Rabbit Monoclonal Antibody [PSH24-09]
cat.: HA724421
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH24-09 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse CXCL10 / IP-10 aa 22-98 (HA210805). |
| Positive control: | Recombinant Mouse CXCL10 / IP-10 protein (HA210805). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-08] to Mouse CXCL10 / IP-10 (Capture) (HA724420) and Recombinant Mouse CXCL10 / IP-10 protein (HA210805) as the standard. The reference range value is 15.6-2,000 pg/mL. |
| Uniprot #: | SwissProt: P17515 Mouse |
| Alternative names: | C-X-C motif chemokine 10 10 kDa interferon gamma-induced protein (Gamma-IP10; IP-10) C7 Interferon-gamma induced protein CRG-2 Small-inducible cytokine B10 Cxcl10 Crg2 Ifi10 Inp10 Scyb10 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse CXCL10 / IP-10 matched pair antibodies Capture: HA724420, Mouse CXCL10 / IP-10 Rabbit mAb [PSH24-08] Detector: HA724421, Mouse CXCL10 / IP-10 Rabbit mAb [PSH24-09] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724420) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CXCL10 / IP-10 protein (HA210805) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA724421, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CXCL10 / IP-10 in LPS unstimulated and stimulated RAW264.7 cell culture supernatant. Capture: HA724420, Mouse CXCL10 / IP-10 Rabbit mAb [PSH24-08] Detector: HA724421, Mouse CXCL10 / IP-10 Rabbit mAb [PSH24-09] RAW264.7 cells were stimulated with 1 µg/ml LPS or vehicle control and incubated for 4 days. The concentrations of CXCL10 / IP-10 measured in duplicate and interpolated from the CXCL10 / IP-10 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 25% and stimulated 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL10 / IP-10 concentration was determined to be 1,437 pg/ml in LPS stimulated RAW264.7 cell culture supernatant and 282 pg/ml in the unstimulated RAW264.7 control. |
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Fig3:
Interpolated concentrations of spiked CXCL10 / IP-10 in mouse cell culture media samples. Capture: HA724420, Mouse CXCL10 / IP-10 Rabbit mAb [PSH24-08] Detector: HA724421, Mouse CXCL10 / IP-10 Rabbit mAb [PSH24-09] The concentrations of CXCL10 / IP-10 were measured in duplicates, interpolated from the CXCL10 / IP-10 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.