CD27 Recombinant Rabbit Monoclonal Antibody [PSH24-12]
cat.: HA724423
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC(Intra) |
| Clonality: | Monoclonal |
| Clone number: | PSH24-12 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC(Intra) |
1:10,000 1:2,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: P26842 Human |
| Alternative names: | CD 27 CD27 CD27 antigen CD27 molecule CD27_HUMAN CD27L receptor LPFS2 MGC20393 OTTHUMP00000238557 S152 T cell activation antigen CD27 T cell antivation antigen S152 T-cell activation antigen CD27 T14 TNFRSF 7 TNFRSF7 TNFSF7 Tp 55 Tp55 Tumor necrosis factor receptor superfamily member 7 |
Images
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Fig1:
Western blot analysis of CD27 on different lysates with Rabbit anti-CD27 antibody (HA724423) at 1/10,000 dilution. Lane 1: Raji (Human Burkitt's lymphoma cells) cell lysate Lane 2: MCF7 (Human breast cancer cells) cell lysate Lane 3: Ramos (Human Burkitt's lymphoma cells) cell lysate Lane 4: Daudi (Human Burkitt's lymphoma cells) cell lysate Lane 5: THP-1 (Human acute monoblastic leukemia cells) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 12 seconds; ECL: K1801 MCF7 and THP-1 are negative controls (PMID: 11062504). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724423, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 29 kDa Observed band size: 50 kDa |
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Fig2:
Application: Immunocytochemistry (IF-cell)
Species: Human Sample: Raji (Human Burkitt's lymphoma cells) / MCF7 (Human breast cancer cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA724423, 1/2,000, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). Negative control: MCF7 (PMID: 11062504). |
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Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724423, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Lymph nodes Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724423, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Human Tissue: Skeletal muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724423, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative expression of CD27 in human skeletal muscle is consistent with the predicted expression pattern. |
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Fig6:
Application: Flow Cytometry (Intra)
Species: Human Sample: Raji (Human Burkitt's lymphoma cells) / MCF7 (Human breast cancer cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 1x Permeabilization Buffer (eBioscience), 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: HA724423 (1/1,000) (Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. Negative control: MCF7 (PMID: 11062504). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.