ROR alpha Recombinant Rabbit Monoclonal Antibody [PSH24-13]
cat.: HA724424
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH24-13 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Positive control: | SH-SY5Y (Human neuroblastoma cells) cell lysate, Mouse cerebellum tissue lysate, Mouse spleen tissue lysate, Rat cerebellum tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:500 |
| Uniprot #: | SwissProt: P35398 Human | P51448 Mouse Entrez Gene: 300807 Rat |
| Alternative names: | fhl2a NR1F1 Nuclear receptor ROR alpha Nuclear receptor ROR-alpha Nuclear receptor RZR-alpha Nuclear receptor subfamily 1 group F member 1 RAR related orphan receptor A RAR related orphan receptor alpha RAR-related orphan receptor A Retinoid-related orphan receptor-alpha Rora RORA_HUMAN RZR alpha RZR-ALPHA RZRA Transcription factor RZR alpha |
Images
|
Fig1:
Western blot analysis of ROR alpha on different lysates with Rabbit anti-ROR alpha antibody (HA724424) at 1/2,000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma cells) cell lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: Rat cerebellum tissue lysate Lane 5: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; ECL: K1801 Low expression: Spleen (PMID: 9226375). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724424, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 59 kDa Observed band size: 59 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724424, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Rat Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724424, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724424, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Low expression of ROR alpha in mouse kidney is consistent with the predicted expression pattern. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.