SLC12A3 Recombinant Rabbit Monoclonal Antibody [PSH24-16]
cat.: HA724427
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-Fr, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH24-16 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 111 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse SLC12A3 aa 1-133. |
| Positive control: | Mouse kidney (Unboiled) tissue lysate, Rat kidney (Unboiled) tissue lysate. |
| Subcellular location: | Cell membrane, Apical cell membrane. |
| Recommended Dilutions:
WB IHC-Fr IHC-P IP |
1:5,000 1:1,000 1:90,000 1-2μg/sample |
| Uniprot #: | SwissProt: P59158 Mouse | P55018 Rat |
| Alternative names: | Solute carrier family 12 member 3 Na-Cl symporter Thiazide-sensitive sodium-chloride cotransporter Slc12a3 Ncc Tsc |
Images
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Fig1:
Western blot analysis of SLC12A3 on different lysates with Rabbit anti-SLC12A3 antibody (HA724427) at 1/5,000 dilution.
Lane 1: Mouse kidney (Unboiled) tissue lysate Lane 2: Mouse lung (Unboiled) tissue lysate Lane 3: Mouse liver (Unboiled) tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 14 seconds; ECL: K1801 Negative control: lung and liver (PMID: 8812482). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724427, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 111 kDa Observed band size: 150 kDa |
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Fig2:
Western blot analysis of SLC12A3 on different lysates with Rabbit anti-SLC12A3 antibody (HA724427) at 1/5,000 dilution.
Lane 1: Rat kidney (Unboiled) tissue lysate Lane 2: Rat lung (Unboiled) tissue lysate Lane 3: Rat liver (Unboiled) tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 18 seconds; ECL: K1801 Negative control: lung and liver (PMID: 8812482). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724427, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 111 kDa Observed band size: 150 kDa |
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Fig3:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Kidney Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724427, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig4:
Application: Immunofluorescence (IHC-Fr)
Species: Rat Tissue: Kidney Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724427, 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724427, 1/90,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig6:
Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Lung Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724427, 1/90,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative control: lung (PMID: 8812482). |
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Fig7:
Application: Immunohistochemistry (IHC-P) Species: Rat Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724427, 1/90,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig8:
Immunoprecipitation (IP)
SLC12A3 was immunoprecipitated in 0.2 mg mouse kidney tissue lysate with HA724427 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724427 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse kidney tissue lysate (input) Lane 2: HA724427 IP in mouse kidney tissue lysate Lane 3: Rabbit IgG instead of HA724427 in mouse kidney tissue lysate Exposure time: 2 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724427, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 111 kDa Observed band size: 150 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.