Mouse CD25 / IL-2 Receptor alpha Recombinant Rabbit Monoclonal Antibody [PSH24-24]
cat.: HA724431
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH24-24 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse IL-2 Receptor alpha aa 22-236 (HA211117). |
| Positive control: | Recombinant Mouse IL-2 Receptor alpha protein (HA211117). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-25] to Mouse IL-2 Receptor alpha (Detecor) (HA724432) and Recombinant Mouse IL-2 Receptor alpha protein (HA211117) as the standard. The reference range value is 23.4-3,000 pg/mL. |
| Uniprot #: | SwissProt: P01590 Mouse |
| Alternative names: | Interleukin 2 receptor alpha chain CD25 CD25 antigen IDDM10 IL 2 receptor alpha subunit IL-2 receptor subunit alpha IL-2-RA IL-2R subunit alpha IL2 RA IL2 Receptor alpha IL2-RA IL2R IL2R, alpha chain IL2RA IMD41 Interleukin 2 receptor alpha Interleukin 2 receptor Interleukin-2 receptor subunit alpha p55 t-cell growth factor receptor TAC TAC antigen TCGFR |
Images
|
Fig1:
Sandwich ELISA analysis of Mouse IL-2 Receptor alpha matched pair antibodies Capture: HA724431, Mouse IL-2 Receptor alpha Rabbit mAb [PSH24-24] Detector: HA724432, Mouse IL-2 Receptor alpha Rabbit mAb [PSH24-25] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724431) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IL-2 Receptor alpha protein (HA211117) starting from 3,000 pg/ml to 0 pg/ml and detect antibody (HA724432, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native IL-2 Receptor alpha in CTLL-2 and C2C12 cell culture supernatant. Capture: HA724431, Mouse IL-2 Receptor alpha Rabbit mAb [PSH24-24] Detector: HA724432, Mouse IL-2 Receptor alpha Rabbit mAb [PSH24-25] The concentrations of IL-2 Receptor alpha were measured in duplicates, interpolated from the IL-2 Receptor alpha standard curve and corrected for sample dilution. Undiluted samples are CTLL-2 cell culture supernatant 2.5% and C2C12 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2 Receptor alpha concentration was determined to be 52.3 ng/ml in CTLL-2 cell culture supernatant and undetectable in C2C12 cell culture supernatant. |
|
Fig3:
Interpolated concentrations of native IL-2 Receptor alpha in mouse serum samples. Capture: HA724431, Mouse IL-2 Receptor alpha Rabbit mAb [PSH24-24] Detector: HA724432, Mouse IL-2 Receptor alpha Rabbit mAb [PSH24-25] The concentrations of IL-2 Receptor alpha were measured in duplicates, interpolated from the IL-2 Receptor alpha standard curve and corrected for sample dilution. Undiluted samples are mouse serum 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2 Receptor alpha concentration was determined to be 6,004 pg/ml in mouse serum. |
|
Fig4:
Interpolated concentrations of spiked IL-2 Receptor alpha in mouse cell culture media samples. Capture: HA724431, Mouse IL-2 Receptor alpha Rabbit mAb [PSH24-24] Detector: HA724432, Mouse IL-2 Receptor alpha Rabbit mAb [PSH24-25] The concentrations of IL-2 Receptor alpha were measured in duplicates, interpolated from the IL-2 Receptor alpha standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.