HRP Conjugated Human/Monkey Apolipoprotein CIII Recombinant Rabbit Monoclonal Antibody [PSH24-27]
cat.: HA724434H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rhesus monkey |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH24-27 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Apolipoprotein CIII aa 21-99 (HA211274). |
| Positive control: | Recombinant Human Apolipoprotein CIII protein (HA211274). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-26] to Human/Monkey Apolipoprotein CIII (Capture) (HA724433) and Recombinant Human Apolipoprotein CIII protein (HA211274) as the standard. The reference range value is 156.25-10,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P02656 Human |
| Alternative names: | APOC3 APO C3 Apo CIII Apo-CIII APOC 3 ApoC III ApoC-III APOC3 APOC3_HUMAN ApoCIII Apolipoprotein C III Apolipoprotein C-III Apolipoprotein C3 ApolipoproteinCIII MGC150353 |
Images
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Fig1:
Sandwich ELISA analysis of Human Apolipoprotein CIII matched pair antibodies Capture: HA724433, Human/Monkey Apolipoprotein CIII Rabbit mAb [PSH24-26] Detector: HA724434, Human/Monkey Apolipoprotein CIII Rabbit mAb [PSH24-27] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724433) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Apolipoprotein CIII protein (HA211274) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724434, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.