Human/Monkey Apo-M Recombinant Rabbit Monoclonal Antibody [PSH24-28]
cat.: HA724435
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rhesus monkey |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH24-28 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Apo-M aa 23-188 (HA211307). |
| Positive control: | Recombinant Human Apo-M protein (HA211307). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-29] to Human/Monkey Apo-M (Detecor) (HA724436) and Recombinant Human Apo-M protein (HA211307) as the standard. The reference range value is 7.8-2,000 pg/mL. |
| Uniprot #: | SwissProt: O95445 Human |
| Alternative names: | Apo M Apo-M Apolipoprotein M ApoM APOM_HUMAN G3A HSPC336 MGC22400 NG20 NG20 like protein Protein G3a |
Images
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Fig1:
Sandwich ELISA analysis of Human Apo-M matched pair antibodies Capture: HA724435, Human/Monkey Apo-M Rabbit mAb [PSH24-28] Detector: HA724436, Human/Monkey Apo-M Rabbit mAb [PSH24-29] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724435) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Apo-M protein (HA211307) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA724436, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Apo-M in HepG2 cell culture supernatant. Capture: HA724435, Human/Monkey Apo-M Rabbit mAb [PSH24-28] Detector: HA724436, Human/Monkey Apo-M Rabbit mAb [PSH24-29] The concentrations of Apo-M were measured in duplicates, interpolated from the Apo-M standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-M concentration was determined to be 6,910 pg/ml in HepG2 cell culture supernatant. |
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Fig3:
Interpolated concentrations of native Apo-M in human serum samples. Capture: HA724435, Human/Monkey Apo-M Rabbit mAb [PSH24-28] Detector: HA724436, Human/Monkey Apo-M Rabbit mAb [PSH24-29] The concentrations of Apo-M were measured in duplicates, interpolated from the Apo-M standard curve and corrected for sample dilution. Undiluted samples are human serum 0.0025%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-M concentration was determined to be 23.5 μg/ml in human serum. |
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Fig4:
Interpolated concentrations of native Apo-M in Rhesus monkey serum samples. Capture: HA724435, Human/Monkey Apo-M Rabbit mAb [PSH24-28] Detector: HA724436, Human/Monkey Apo-M Rabbit mAb [PSH24-29] The concentrations of Apo-M were measured in duplicates, interpolated from the Apo-M standard curve and corrected for sample dilution. Undiluted samples are Rhesus monkey serum 0.05%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-M concentration was determined to be 1,847 ng/ml in Rhesus monkey serum. |
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Fig5:
Interpolated concentrations of spiked Apo-M in human cell culture media samples. Capture: HA724435, Human/Monkey Apo-M Rabbit mAb [PSH24-28] Detector: HA724436, Human/Monkey Apo-M Rabbit mAb [PSH24-29] The concentrations of Apo-M were measured in duplicates, interpolated from the Apo-M standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.