Biotin Conjugated Human/Monkey Apo-M Recombinant Rabbit Monoclonal Antibody [PSH24-29]
cat.: HA724436B
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rhesus monkey
Applications: ELISA(Det), ELISA
Clonality: Monoclonal
Clone number: PSH24-29
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG
Immunogen: Recombinant protein within Human Apo-M aa 23-188 (HA211307).
Positive control: Recombinant Human Apo-M protein (HA211307).
Subcellular location: Secreted.
Recommended Dilutions:
  ELISA(Det)
  ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-28] to Human/Monkey Apo-M (Capture) (HA724435) or Rabbit monoclonal [PSH24-30] to Human/Monkey Apo-M (Capture) (HA724437) and Recombinant Human Apo-M protein (HA211307) as the standard. The reference range value is 7.8-2,000 pg/mL.
Use at an assay dependent concentration.
Uniprot #: SwissProt: O95445 Human
Alternative names: Apo M Apo-M Apolipoprotein M ApoM APOM_HUMAN G3A HSPC336 MGC22400 NG20 NG20 like protein Protein G3a
Images
HA724436B_1.png Fig1: Sandwich ELISA analysis of Human Apo-M matched pair antibodies

Capture: HA724435, Human/Monkey Apo-M Rabbit mAb [PSH24-28]
Detector: HA724436, Human/Monkey Apo-M Rabbit mAb [PSH24-29]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724435) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Apo-M protein (HA211307) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA724436, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA724436B_2.png Fig2: Sandwich ELISA analysis of Human Apo-M matched pair antibodies

Capture: HA724437, Human/Monkey Apo-M Rabbit mAb [PSH24-30]
Detector: HA724436, Human/Monkey Apo-M Rabbit mAb [PSH24-29]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724437) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Apo-M protein (HA211307) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA724436, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA724436B_3.png Fig3: Interpolated concentrations of native Apo-M in HepG2 cell culture supernatant.

Capture: HA724435, Human/Monkey Apo-M Rabbit mAb [PSH24-28]
Detector: HA724436, Human/Monkey Apo-M Rabbit mAb [PSH24-29]

The concentrations of Apo-M were measured in duplicates, interpolated from the Apo-M standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-M concentration was determined to be 6,910 pg/ml in HepG2 cell culture supernatant.
HA724436B_4.png Fig4: Interpolated concentrations of native Apo-M in HepG2 cell culture supernatant.

Capture: HA724437, Human/Monkey Apo-M Rabbit mAb [PSH24-30]
Detector: HA724436, Human/Monkey Apo-M Rabbit mAb [PSH24-29]

The concentrations of Apo-M were measured in duplicates, interpolated from the Apo-M standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-M concentration was determined to be 9,538 pg/ml in HepG2 cell culture supernatant.
HA724436B_5.png Fig5: Interpolated concentrations of native Apo-M in human serum samples.

Capture: HA724435, Human/Monkey Apo-M Rabbit mAb [PSH24-28]
Detector: HA724436, Human/Monkey Apo-M Rabbit mAb [PSH24-29]

The concentrations of Apo-M were measured in duplicates, interpolated from the Apo-M standard curve and corrected for sample dilution. Undiluted samples are human serum 0.0025%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-M concentration was determined to be 23.5 μg/ml in human serum.
HA724436B_6.png Fig6: Interpolated concentrations of native Apo-M in human serum samples.

Capture: HA724437, Human/Monkey Apo-M Rabbit mAb [PSH24-30]
Detector: HA724436, Human/Monkey Apo-M Rabbit mAb [PSH24-29]

The concentrations of Apo-M were measured in duplicates, interpolated from the Apo-M standard curve and corrected for sample dilution. Undiluted samples are human serum 0.005%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-M concentration was determined to be 34.3 μg/ml in human serum.
HA724436B_7.png Fig7: Interpolated concentrations of native Apo-M in Rhesus monkey serum samples.

Capture: HA724435, Human/Monkey Apo-M Rabbit mAb [PSH24-28]
Detector: HA724436, Human/Monkey Apo-M Rabbit mAb [PSH24-29]

The concentrations of Apo-M were measured in duplicates, interpolated from the Apo-M standard curve and corrected for sample dilution. Undiluted samples are Rhesus monkey serum 0.05%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-M concentration was determined to be 1,847 ng/ml in Rhesus monkey serum.
HA724436B_8.png Fig8: Interpolated concentrations of native Apo-M in Rhesus monkey serum samples.

Capture: HA724437, Human/Monkey Apo-M Rabbit mAb [PSH24-30]
Detector: HA724436, Human/Monkey Apo-M Rabbit mAb [PSH24-29]

The concentrations of Apo-M were measured in duplicates, interpolated from the Apo-M standard curve and corrected for sample dilution. Undiluted samples are Rhesus monkey serum 0.05%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apo-M concentration was determined to be 962 ng/ml in Rhesus monkey serum.
HA724436B_9.png Fig9: Interpolated concentrations of spiked Apo-M in human cell culture media samples.

Capture: HA724435, Human/Monkey Apo-M Rabbit mAb [PSH24-28]
Detector: HA724436, Human/Monkey Apo-M Rabbit mAb [PSH24-29]

The concentrations of Apo-M were measured in duplicates, interpolated from the Apo-M standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
HA724436B_10.png Fig10: Interpolated concentrations of spiked Apo-M in human cell culture media samples.

Capture: HA724437, Human/Monkey Apo-M Rabbit mAb [PSH24-30]
Detector: HA724436, Human/Monkey Apo-M Rabbit mAb [PSH24-29]

The concentrations of Apo-M were measured in duplicates, interpolated from the Apo-M standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.