Human/Monkey ESM1 Recombinant Rabbit Monoclonal Antibody [PSH24-31]
cat.: HA724438
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rhesus monkey |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH24-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ESM1 aa 20-184(R44A,R45A,S156A) (HA211552). |
| Positive control: | Recombinant Human ESM1 protein (HA211552). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-32] to Human/Monkey ESM1 (Detecor) (HA724439) and Recombinant Human ESM1 protein (HA211552) as the standard. The reference range value is 15.6-4,000 pg/mL. |
| Uniprot #: | SwissProt: Q9NQ30 Human |
| Alternative names: | Endocan Endothelial cell specific molecule 1 Endothelial cell-specific molecule 1 ESM 1 ESM 1 secretory protein ESM-1 Esm1 ESM1 secretory protein ESM1_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of Human ESM1 matched pair antibodies Capture: HA724438, Human/Monkey ESM1 Rabbit mAb [PSH24-31] Detector: HA724439, Human/Monkey ESM1 Rabbit mAb [PSH24-32] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724438) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ESM1 protein (HA211552) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA724439, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native ESM1 in EA.hy926 cell culture supernatant. Capture: HA724438, Human/Monkey ESM1 Rabbit mAb [PSH24-31] Detector: HA724439, Human/Monkey ESM1 Rabbit mAb [PSH24-32] Interpolated concentration of native ESM1 was measured in duplicate at different sample concentrations and interpolated from the ESM1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ESM1 concentration was determined to be 2,431 pg/ml in EA.hy926 cell culture supernatant. |
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Fig3:
Interpolated concentrations of native ESM1 in human serum. Capture: HA724438, Human/Monkey ESM1 Rabbit mAb [PSH24-31] Detector: HA724439, Human/Monkey ESM1 Rabbit mAb [PSH24-32] Interpolated concentration of native ESM1 was measured in duplicate at different sample concentrations and interpolated from the ESM1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ESM1 concentration was determined to be 1,068 pg/ml in Human serum. |
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Fig4:
Interpolated concentrations of native ESM1 in rhesus monkey serum. Capture: HA724438, Human/Monkey ESM1 Rabbit mAb [PSH24-31] Detector: HA724439, Human/Monkey ESM1 Rabbit mAb [PSH24-32] Interpolated concentration of native ESM1 was measured in duplicate at different sample concentrations and interpolated from the ESM1 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean ESM1 concentration was determined to be 1,204 pg/ml in rhesus monkey serum. |
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Fig5:
Interpolated concentrations of spiked ESM1 in cell culture media samples. Capture: HA724438, Human/Monkey ESM1 Rabbit mAb [PSH24-31] Detector: HA724439, Human/Monkey ESM1 Rabbit mAb [PSH24-32] The concentrations of ESM1 were measured in duplicates, interpolated from the ESM1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.