PAI1 Recombinant Rabbit Monoclonal Antibody [PSH24-33]
cat.: HA724440
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Green monkey |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH24-33 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PAI1 aa 1-402. |
| Positive control: | A549 treated with 5ng/ml hTGFβ1 for 48 hours and 300ng/ml BFA for 16 hours cell lysate, EA.hy926 (Human umbilical vein fusion cells) cell lysate, COS-1 (African green monkey kidney fibroblasts) cell lysate, Mouse placenta (E17) tissue lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IP |
1:5,000 1-2μg/sample |
| Uniprot #: | SwissProt: P05121 Human | P22777 Mouse | P20961 Rat |
| Alternative names: | Clade E Endothelial plasminogen activator inhibitor Nexin Nexin plasminogen activator inhibitor type 1 PAI 1 PAI PAI-1 PAI1_HUMAN PLANH1 Plasminogen activator inhibitor 1 Plasminogen activator inhibitor type 1 Serine (or cysteine) proteinase inhibitor Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 Serine proteinase inhibitor clade E member 1 serpin Serpin E1 Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 Serpin peptidase inhibitor clade E Serpine 1 SERPINE1 |
Images
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Fig1:
Western blot analysis of PAI1 on different lysates with Rabbit anti-PAI1 antibody (HA724440) at 1/5,000 dilution.
Lane 1: A549 (Human lung adenocarcinoma cells) cell lysate Lane 2: A549 treated with 5ng/ml hTGFβ1 for 48 hours and 300ng/ml BFA for 16 hours cell lysate Lane 3: EA.hy926 (Human umbilical vein fusion cells) cell lysate Lane 4: HCT 116 (Human colon cancer cells) cell lysate Lane 5: COS-1 (African green monkey kidney fibroblasts) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 30 seconds; ECL: K1801 HCT 116 is a negative control (PMID: 32344898). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724440, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted MW: 45 kDa Observed MW: 45 kDa |
|
Fig2:
Western blot analysis of PAI1 on different lysates with Rabbit anti-PAI1 antibody (HA724440) at 1/5,000 dilution.
Lane 1: Mouse placenta (E17) tissue lysate Lane 2: Mouse liver tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Mouse liver is a Low expression (PMID: 21898503). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724440, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted MW: 45 kDa Observed MW: 45 kDa |
|
Fig3:
Immunoprecipitation (IP)
PAI1 was immunoprecipitated in 0.2 mg EA.hy926 (Human umbilical vein fusion cell) cell lysate with HA724440 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724440 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: EA.hy926 cell lysate (input) Lane 2: HA724440 IP in EA.hy926 cell lysate Lane 3: Rabbit IgG instead of HA724440 in EA.hy926 cell lysate Exposure time: 5 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724440, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted MW: 45 kDa Observed MW: 45 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.