Periostin Recombinant Rabbit Monoclonal Antibody [PSH24-35]
cat.: HA724442
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-Fr, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH24-35 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Periostin aa 1-838. |
| Positive control: | Mouse skin tissue lysate, Mouse spleen tissue lysate, Mouse skeletal muscle tissue lysate, Mouse stomach tissue lysate. |
| Subcellular location: | Golgi apparatus, Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IHC-Fr IHC-P |
1:5,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: Q62009 Mouse |
| Alternative names: | Fasciclin-I like MGC119510 MGC119511 OSF 2 OSF-2 OSF2 Osteoblast specific factor 2 (fasciclin I like) Osteoblast specific factor 2 Osteoblast specific factor Osteoblast-specific factor 2 PDLPOSTN Periodontal ligament specific periostin Periostin Periostin isoform thy2 Periostin isoform thy4 Periostin isoform thy6 Periostin isoform thy8 Periostin osteoblast specific factor PN POSTN RP11 412K4.1 |
Images
|
Fig1:
Western blot analysis of Periostin on different lysates with Rabbit anti-Periostin antibody (HA724442) at 1/5,000 dilution.
Lane 1: Mouse skin tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Mouse skeletal muscle tissue lysate Lane 4: Mouse stomach tissue lysate Low expression: Mouse spleen (PMID: 18086302), Mouse skeletal muscle (PMID: 15731169) Lysates/proteins at 30 µg/Lane. Exposure time: 43 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724442, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 93 kDa Observed band size: 85 kDa |
|
Fig2:
Application: Immunofluorescence (IHC-Fr)
Species: Mouse Tissue: Stomach Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724442, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Skin Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724442, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Stomach Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724442, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Skeletal muscle Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724442, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Low expression: Mouse skeletal muscle (PMID: 15731169) |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.