Calpain 1 Recombinant Rabbit Monoclonal Antibody [PSH24-37]
cat.: HA724444
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Green monkey |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH24-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 82 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Calpain 1 aa 551-600. |
| Positive control: | A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, MDA-MB-231 (Human breast cancer cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, Jurkat (Human T-lymphoblastic cells) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, C6 (Rat glioma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate. |
| Subcellular location: | Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P07384 Human | O35350 Mouse | P97571 Rat |
| Alternative names: | Ca2 activated neutral protease Calcium activated neutral proteinase Calcium activated neutral proteinase small subunit Calcium dependent protease small subunit 1 Calcium dependent protease small subunit Calcium-activated neutral proteinase 1 Calpain 1 Calpain 1 large subunit Calpain 1, (mu/I) large subunit Calpain mu type Calpain mu-type Calpain regulatory subunit Calpain small subunit 1 Calpain, large polypeptide L1 Calpain-1 catalytic subunit Calpain-1 large subunit CAN1_HUMAN CANP 1 CANP CANP small subunit CANP1 CANPL 1 CANPL1 CAPN 1 CAPN1 Cell proliferation inducing protein 30 Cell proliferation-inducing gene 30 protein Micromolar Calpain Micromolar-calpain Mu Calpain muCANP muCL |
Images
|
Fig1:
Western blot analysis of Calpain 1 on different lysates with Rabbit anti-Calpain 1 antibody (HA724444) at 1/1,000 dilution.
Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 2: MDA-MB-231 (Human breast cancer cell) cell lysate Lane 3: MCF7 (Human breast cancer cell) cell lysate Lane 4: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 5: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 6: NIH/3T3 (Mouse fibroblast) cell lysate Lane 7: C6 (Rat glioma cell) cell lysate Lane 8: COS-1 (African green monkey kidney fibroblast) cell lysate Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724444, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 82 kDa Observed band size: 70 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.