MTBR-TAU 243 lsoform 2N4R (225-242) Recombinant Rabbit Monoclonal Antibody [PSH24-42]
cat.: HA724446
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Pig, Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH24-42 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MAPT aa 225-242. |
| Positive control: | Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, Rat cerebellum tissue lysate, Pig brain tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, Cell membrane, cytoskeleton, Cell projection, axon, dendrite, Secreted. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:80,000 1-2μg/sample |
| Uniprot #: | SwissProt: P10637 Mouse | P19332 Rat |
| Alternative names: | AI413597 AW045860 DDPAC FLJ31424 FTDP 17 G protein beta1/gamma2 subunit interacting factor 1 MAPT MAPTL MGC134287 MGC138549 MGC156663 Microtubule associated protein tau Microtubule associated protein tau isoform 4 Microtubule-associated protein tau MSTD Mtapt MTBT1 MTBT2 Neurofibrillary tangle protein Paired helical filament tau Paired helical filament-tau PHF tau PHF-tau PPND PPP1R103 Protein phosphatase 1, regulatory subunit 103 pTau RNPTAU TAU Tauopathy and respiratory failure, included |
Images
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Fig1:
Western blot analysis of MTBR-TAU 243 lsoform 2N4R (225-242) on different lysates with Rabbit anti-MTBR-TAU 243 lsoform 2N4R (225-242) antibody (HA724446) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Mouse cerebellum tissue lysate Lane 4: Mouse testis tissue lysate Lane 5: Rat brain tissue lysate Lane 6: Rat spleen tissue lysate Lane 7: Rat cerebellum tissue lysate Lane 8: Rat testis tissue lysate Lane 9: Pig brain tissue lysate Lane 10: Pig spleen tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 7 seconds; ECL: K1801 Negative control: spleen and testis (PMID : 24309898; PMID : 24386422). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724446, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Observed band size: 50-80 kDa |
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Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Alzheimer's brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724446, 1/80,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Brain Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724446, 1/80,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig4:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Cerebellum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724446, 1/80,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig5:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724446, 1/80,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. Negative control: Spleen (PMID: 24309898; PMID: 24386422). |
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Fig6:
Immunoprecipitation (IP)
MTBR-TAU 243 lsoform 2N4R (225-242) was immunoprecipitated in 0.2 mg mouse brain tissue lysate with HA724446 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724446 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: HA724446 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA724446 in mouse brain tissue lysate Exposure time: 6 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724446, 1/10,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Observed band size: 50-80 kDa |
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Fig7:
Immunoprecipitation (IP)
20 ng of full-length TAU isoform 2N4R protein (1-441 aa) and 200 ng of truncated TAU isoform 2N4R protein (1-256 aa) were incubated with 0.2 mg of negative tissue lysate from mouse spleen, along with HA724446 at 2 µg/10 µl of beads. Western blot was performed from the immunoprecipitate using HA724446 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: TAU isoform 2N4R (full-length protein, amino acids 1–441) and TAU isoform 2N4R (truncated protein, amino acids 1–256) were spiked into negative tissue lysate from mouse spleen (input) Lane 2: HA724446 IP in TAU isoform 2N4R (full-length protein, amino acids 1–441) and TAU isoform 2N4R (truncated protein, amino acids 1–256) were spiked into negative tissue lysate from mouse spleen Lane 3: Rabbit IgG instead of HA724446 in TAU isoform 2N4R (full-length protein, amino acids 1–441) and TAU isoform 2N4R (truncated protein, amino acids 1–256) were spiked into negative tissue lysate from mouse spleen Exposure time: 6 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724446, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Observed band size: 50-80 kDa |
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Fig8: HA724446 epitope mapping schematic diagram. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.