Chil3 / Ym-1 Recombinant Rabbit Monoclonal Antibody [PSH24-49]
cat.: HA724451
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH24-49 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse Chil3 aa 22-398. |
| Positive control: | Mouse lung tissue lysate, Mouse bone marrow tissue lysate, Mouse spleen tissue lysate. |
| Subcellular location: | Secreted, Rough endoplasmic reticulum lumen, Nucleus envelope, Cytoplasm, Cytoplasmic granule. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:100,000 1:2,000 1:50 |
| Uniprot #: | SwissProt: O35744 Mouse |
| Alternative names: | Chitinase-like protein 3 EC:3.2.1.52 Beta-N-acetylhexosaminidase Ym1 Chitinase-3-like protein 3 ECF-L Eosinophil chemotactic cytokine Secreted protein Ym1 Chil3 Chi3l3 Ym1 |
Images
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Fig1:
Western blot analysis of Chil3 / Ym-1 on different lysates with Rabbit anti-Chil3 / Ym-1 antibody (HA724451) at 1/100,000 dilution. Lane 1: Mouse lung tissue lysate (30 µg/Lane) Lane 2: Mouse brain tissue lysate (30 µg/Lane) Lane 3: Mouse bone marrow tissue lysate (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (30 µg/Lane) Exposure time: 30 seconds; ECL: K1801 Low expression: Mouse brain (PMID: 25551794). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724451, 1/100,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 44 kDa Observed band size: 44 kDa |
|
Fig2:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Spleen Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724451, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig3:
Application: Immunohistochemistry (IHC-P)
Species: Mouse Tissue: Lung Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724451, 1/2,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
|
Fig4:
Application: Immunofluorescence (IF-tissue)
Species: Mouse Tissue: Lung Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA724451, 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.