Biotin Conjugated Mouse CD28 Recombinant Rabbit Monoclonal Antibody [PSH24-62]
cat.: HA724458B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH24-62 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse CD28 aa 20-150 (HA211340). |
| Positive control: | Recombinant Mouse CD28 protein (HA211340). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-61] to Mouse CD28 (Capture) (HA724457) and Recombinant Mouse CD28 protein (HA211340) as the standard. The reference range value is 7.8-1,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P31041 Mouse |
| Alternative names: | CD 28 CD28 CD28 antigen CD28 molecule CD28_HUMAN MGC138290 T cell antigen CD28 T cell specific surface glycoprotein T cell specific surface glycoprotein CD28 T-cell-specific surfac |
Images
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Fig1:
Sandwich ELISA analysis of Mouse CD28 matched pair antibodies Capture: HA724457, Mouse CD28 Rabbit mAb [PSH24-61] Detector: HA724458, Mouse CD28 Rabbit mAb [PSH24-62] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724457) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CD28 protein (HA211340) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA724458, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CD28 in mouse spleen and pancreas tissue extract samples based on a 1,000 µg/ml extract load. Capture: HA724457, Mouse CD28 Rabbit mAb [PSH24-61] Detector: HA724458, Mouse CD28 Rabbit mAb [PSH24-62] Interpolated concentration of native CD28 was measured in duplicate at different sample concentrations and interpolated from the CD28 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CD28 concentration was determined to be 5,430 pg/mL in mouse spleen tissue extract. There was no detectable signal in mouse pancreas tissue extract. |
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Fig3:
Interpolated concentrations of spiked CD28 in cell culture media samples. Capture: HA724457, Mouse CD28 Rabbit mAb [PSH24-61] Detector: HA724458, Mouse CD28 Rabbit mAb [PSH24-62] The concentrations of CD28 were measured in duplicates, interpolated from the CD28 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.