Mouse VE-Cadherin Recombinant Rabbit Monoclonal Antibody [PSH24-64]
cat.: HA724460
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH24-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse VE-Cadherin aa 46-592 (HA211298). |
| Positive control: | Recombinant Mouse VE-Cadherin protein (HA211298). |
| Subcellular location: | Cell junction, Cell membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-63] to Mouse VE-Cadherin (Capture) (HA724459) and Recombinant Mouse VE-Cadherin protein (HA211298) as the standard. The reference range value is 78.1-20,000 pg/mL. |
| Uniprot #: | SwissProt: P55284 Mouse |
| Alternative names: | 7B 4 7B4 7B4 antigen Cadherin 5 Cadherin 5 type 2 Cadherin 5, type 2 (vascular endothelium) Cadherin 5, type 2, VE cadherin (vascular epithelium) cadherin, vascular endothelial cadherin, vascular endothelial, 1 Cadherin-5 Cadherin5 CD 144 CD144 CD144 antigen CDH 5 CDH5 CDH5 protein Endothelial specific cadherin FLJ17376 OTTHUMP00000174777 Vascular endothelial cadherin Vascular epithelium cadherin VE Cad VE-cadherin VEC |
Images
|
Fig1:
Sandwich ELISA analysis of Mouse VE-Cadherin matched pair antibodies Capture: HA724459, Mouse VE-Cadherin Rabbit mAb [PSH24-63] Detector: HA724460, Mouse VE-Cadherin Rabbit mAb [PSH24-64] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724459) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse VE-Cadherin protein (HA211298) starting from 20,000 pg/mL to 0 pg/mL and detect antibody (HA724460, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native VE-Cadherin in mouse serum samples and C2C12 cell culture supernatant. Capture: HA724459, Mouse VE-Cadherin Rabbit mAb [PSH24-63] Detector: HA724460, Mouse VE-Cadherin Rabbit mAb [PSH24-64] The concentrations of VE-Cadherin were measured in duplicates, interpolated from the VE-Cadherin standard curve and corrected for sample dilution. Undiluted samples are mouse serum 0.25% and C2C12 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Albumin concentration was determined to be 4,926 ng/mL in mouse serum and undetectabel in C2C12 cell culture supernatant. |
|
Fig3:
Interpolated concentrations of native VE-Cadherin in bEnd.3 and C2C12 cell extract samples based on a 1,000 µg/mL extract load. Capture: HA724459, Mouse VE-Cadherin Rabbit mAb [PSH24-63] Detector: HA724460, Mouse VE-Cadherin Rabbit mAb [PSH24-64] The concentrations of VE-Cadherin were measured in duplicates, interpolated from the VE-Cadherin standard curve and corrected for sample dilution. Undiluted samples are bEnd.3 cell extract 50% and C2C12 cell extract 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VE-Cadherin concentration was determined to be 3,430 pg/mL in bEnd.3 cell extract and undetectable in C2C12 cell extract. |
|
Fig4:
Interpolated concentrations of spiked VE-Cadherin in mouse cell culture media samples. Capture: HA724459, Mouse VE-Cadherin Rabbit mAb [PSH24-63] Detector: HA724460, Mouse VE-Cadherin Rabbit mAb [PSH24-64] The concentrations of VE-Cadherin were measured in duplicates, interpolated from the VE-Cadherin standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.